A779 Inhibitor – A 779 3tfa

Solid. Powder

98.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

872.97

<1mg/ml refers to the product slightly soluble or insoluble

C39H60N12O11

OC(=O)C(F)(F)F.CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@@H](N)CC(O)=O)C(C)C)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N[C@H](C)C(O)=O

HUVECs were cultured in vitro and divided into six groups: the control group (normal medium), the ox-LDL group(treated with 75mg/L ox-LDL), the ox-LDL+ Ang-(1-7) group (1uMol/L Ang-(1-7) pretreated for 30 minutes, then intervened with 75mg/L ox-LDL), the ox-LDL+ Ang-(1-7)+ A-779 group(1uMol/L A-779 (Mas receptor) pretreated for 30 minutes, 1uMol/L Ang-(1-7) pretreated for 30 minutes, then intervened with 75mg/L ox-LDL), the ox-LDL+ A-779 group (1uMol/L A-779 pretreated for 30 minutes, then intervened with 75mg/L ox-LDL), the ox-LDL+ HTA125 group (10ug/L HTA125 (TLR4-blocking antibody) pretreated for 30 minutes, then intervened with 75mg/L ox-LDL). The corresponding index was detected after 24 hours after intervention. Apoptosis of cells were detected by Annexin V-FITC/PI double staining flow cytometry and transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL). The generation of reactive oxygen species (ROS), products in oxidative stress, were detected by DCFH-DA staining. The mRNA and protein expression levels of NADPH oxidase 4(NOX4) and TLR4 were detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting analysis respectively[1]. Cell lines: