Pyroxamide Inhibitor

Solid. Powder

98.77%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

265.31

DMSO: 125mg/mL (471.15mM)
(<1mg/ml refers to the product slightly soluble or insoluble)

C13H19N3O3

O=C(CCCCCCC(=O)Nc1cccnc1)NO

382180-17-8

HDAC1: ic50 0.1-0.2uM

HDAC1 enzyme assay, A MEL cell line expressing the epitope DYKDDDDK-tagged HDAC1 was generated. HDAC1-DYKDDDDK was affinity purified by immunoprecipitation using M2 anti-DYKDDDDK antibody-coated agarose, followed by elution from the agarose using the DYKDDDDK peptide. [3H]acetate-labeled cellular histones were prepared from MEL cells and were used as a substrate for the HDAC activity assay. Released [3H]acetic acid was quantified by scintillation counting. For inhibition studies, the enzyme preparations were preincubated with pyroxamide (10 to 100,000nM) for 30 minutes at 4 degree C.[1]

Pyroxamide may be a useful agent for the treatment of malignancy and that induction of p21/WAF1 in transformed cells by pyroxamide may contribute to the antitumor effects of this agent[1].

Chromatin/Epigenetic; HDAC; DNA Damage/DNA Repair