RS3 Inhibitor – Ginsenoside

Solid. Powder

98.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

827.1

<1mg/ml refers to the product slightly soluble or insoluble

C44H74O14

[H][C@@]12[C@H](CC[C@@]1(C)[C@]1(C)CCC3C(C)(C)[C@H](CC[C@]3(C)[C@@]1([H])C[C@H]2O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O)[C@@](C)(O)CC\C=C(/C)C

194861-70-6

p53

Ginsenoside Rs3 (G-Rs3), a new diol-type ginseng saponin isolated from the roots of Panax ginseng C. A. Meyer, efficiently arrests the cell cycle at the G1/S boundary at lower doses, 0.1-5microM, but induces apoptosis at higher doses, 10-25microM, the effects of which were associated with selectively elevating protein levels of p53 and p21WAF1 in SK-HEP-1 cells. The cell growth suppressive and apoptosis inducing effects were confirmed by MTT assays together with flow cytometric analyses, morphological changes and DNA fragmentation. Immunoblotting showed that Ginsenoside Rs3 significantly elevated protein levels of p53 and p21WAF1 prior to inducing apoptosis, while it did not elevate those of cyclin E, cyclin A, p27Kip1, and PCNA. Immune complex kinase assays showed that Ginsenoside Rs3 downregulated the activities of both cyclins E- and A-associated kinases.

Apoptosis; p53