Kumatakenin Inhibitor

Solid. Powder

98.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

314.3

<1mg/ml refers to the product slightly soluble or insoluble

C17H14O6

COc(cc1O)cc(OC(c(cc2)ccc2O)=C2OC)c1C2=O

3301-49-3

Antifection

The methanol extract was re-extracted with hexane, dichloromethane, butanol, and water. A dichloromethane fraction showed a suppressive effect. Suppressive compounds against furylfuramide in the dichloromethane fraction were isolated by SiO(2) column chromatography and identified as 7,4'-di-O-methyleriodictyol (1), 7, 3',4'-tri-O-methyleriodictyol (2), and 3,7,4'-tri-O-methylkaempferol (3). In addition, three flavonoids, ombuine (4), pachypodol (5), and Kumatakenin (6), were isolated and identified from the dichrolomethane fraction. Compounds 1 and 3 suppressed >50% of the SOS-inducing activity at <0.6microMol/mL, and the ID(50) values of both compounds were 0.25microMol/mL. Compound 2 showed a weakly suppressive effect (17%) at a concentration of 0.6microMol/mL, and compounds 4-6 did not. These compounds were also assayed with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which requires liver metabolizing enzymes. Compounds 3,4,5,Kumatakenin (6) suppressed >80% of the SOS-inducing activity of Trp-P-1 at <0.06microMol/mL, and compounds 1 and 2 suppressed 87 and 63% at a concentration of 0.3microMol/mL. In addition, these compounds were assayed with activated Trp-P-1, and the suppressed effects of these compounds were further decreased when compared to Trp-P-1.

Microbiology/Virology; Antifection