Robinetin Inhibitor

Solid. Powder

98.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

302.2

<1mg/ml refers to the product slightly soluble or insoluble

C15H10O7

Oc(cc1)cc(OC(c(cc2O)cc(O)c2O)=C2O)c1C2=O

490-31-3

HIV

Motivated by this scenario, we explored the binding of Robinetin (3,7,3',4',5'-pentahydroxyflavone, a bioflavonoid with remarkable 'two color' intrinsic fluorescence properties), with egg yolk phosphatidylcholine (EYPC) liposomes and normal human hemoglobin (HbA), using steady state and time resolved fluorescence spectroscopy. Distinctive fluorescence signatures obtained for Robinetin indicate its partitioning (K(p)=8.65x10(4)) into the hydrophobic core of the membrane lipid bilayer. HbA-Robinetin interaction was examined using both Robinetin fluorescence and flavonoid-induced quenching of the protein tryptophan fluorescence. Specific interaction with HbA was confirmed from three lines of evidence: (a) bimolecular quenching constant K(q)>>diffusion controlled limit; (b) closely matched values of Stern-Volmer quenching constant and binding constant; (c) tau(0)/tau=1 (where tau(0) and tau are the unquenched and quenched tryptophan fluorescence lifetimes, respectively).

Microbiology/Virology; HIV Protease