Scutebarbatine A Inhibitor

Solid. Powder

98.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

558.6

<1mg/ml refers to the product slightly soluble or insoluble

C32H34N2O7

[H][C@]12CCC=C(C)[C@]1(C)[C@@H](OC(=O)c1cccnc1)[C@H](OC(=O)c1cccnc1)[C@](C)(O)[C@]2(C)\C=C\C1=CC(=O)OC1

176520-13-1

Caspase
Bcl-2

The cytotoxic effect of Scutebarbatine A on A549 in vitro were determined by an MTT assay and evaluated by IC50 values. Furthermore, results of Hoechst 33258 and Annexin V/PI staining assays demonstrated that Scutebarbatine A had significant antitumor effects on A549 cells via apoptosis, in a concentration-dependent manner. What's more, the mechanism was explored by western blotting, and our study revealed that Scutebarbatine A can up-regulate the expressions of cytochrome c, caspase-3 and 9, and down-regulate the levels of Bcl-2 in A549 cells. Finally, the antitumor effects of Scutebarbatine A were evaluated in vivo by using transplanted tumor nude mice, and the results confirmed that Scutebarbatine A has a notable antitumor effect on A549 cancer via mitochondria-mediated apoptosis.

Apoptosis; Caspase