Fagomine Inhibitor

Name: Fagomine Inhibitor; MyBioSource
Brand: MyBioSource
SKU: MBS5764546
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5764546

Fagomine is a glycosidase inhibitor. The Ki of the iminosugar Fagomine is 70uM, 39 uM, and 4.8 uM for Isomaltase (yeast), beta-Glucosidase (bovine), and Amyloglucosidase (A.niger), respectively. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Fagomine, Inhibitor

Full Product Name

Fagomine

Product Synonym Names

D-Fagomine

Form/Format

Solid. Powder

Purity/Purification

98.00%

Preparation and Storage

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

Molecular Weight

147.17

Solubility

Water: 35mg/mL (237.82mM)
(<1mg/ml refers to the product slightly soluble or insoluble)

Formula

C6H12NO3

SMILES

O[C@@H]1CCN[C@@H]([C@]1O)CO

CAS Number

53185-12-9

Cell Research

Human skin fibroblasts from a healthy and three Gaucher disease patients (with N188S/G183W, V230G/R296X, and L444P/L444P mutations) are maintained in DMEM supplemented with 10% FBS as the culture medium. For enzyme activity enhancement assay, cells are cultured in the presence of different concentrations of Fagomine or DMSO alone (as a control) for 5 days and harvested by scraping. Cytotoxicity of Fagomine is monitored by measuring the lactate dehydrogenase activities in the cultured supernatants [1].

Kinase Assay

Lysosomal enzyme activities in cell lysates are determined. Briefly, cells are scraped in ice-cold 0.1% Triton X-100 in water. After centrifugation (6000 rpm for 15 minutes at 4 degree C) to remove insoluble materials, protein concentrations are determined using Protein Assay Rapid Kit. The lysates are incubated at 37 degree C with the corresponding 4-methylumbelliferyl beta-D-glycopyranoside solution in 0.1 M citrate buffer (pH 4). The liberated 4-methylumbelliferone is measured with a fluorescence plate reader (excitation 340nM; emission 460nM). For enzyme inhibition assay, cell lysates from normal skin fibroblasts are mixed with the 4-methylumbelliferyl beta-D-glycopyranoside substrates in the absence or presence of increasing concentrations of Fagomine [1].

In Vitro

Compare to the standard group, rats fed a high-fat high-sucrose diet (HFHS) with Fagomine gain significantly less weight (15.3%) than those fed HFHS (20.9%) [2]. Fagomine counteracts the short-term effects of a high-energy-dense diet on body weight, fasting blood glucose levels and the proportion of gut Enterobacteriales [3].

Product Categories/Family for Fagomine, inhibitor

Metabolism

Product References and Citations for Fagomine, inhibitor

Mena-Barragan T, et al. Inhibitor versus chaperone behaviour of d-fagomine, DAB and LAB sp2-iminosugar conjugates againstglycosidases: A structure-activity relationship study in Gaucher fibroblasts. Eur J Med Chem. 2015 Aug 3pii: S0223-5234(15)30222-1.
Mena-Barragan T, et al. Inhibitor versus chaperone behaviour of d-fagomine, DAB and LAB sp2-iminosugar conjugates againstglycosidases: A structure-activity relationship study in Gaucher fibroblasts. Eur J Med Chem. 2015 Aug 31. pii: S0223-5234(15)30222-1.
Ramos-Romero S, et al. Effect of (D)-fagomine on excreted Enterobacteria and weight gain in rats fed a high-fat high-sucrose diet. Obesity (Silver Spring). 2014 Apr;22(4):976-9.
Ramos-Romero S, et al. Effect of (D)-fagomine on excreted Enterobacteria and weight gain in rats fed a high-fat high-sucrose diet. Obesity (Silver Spring). 2014 Apr;22(4):976-9.
Molinar-Toribio E, et al. D-Fagomine attenuates metabolic alterations induced by a high-energy-dense diet in rats. Food Funct. 2015 Aug;6(8):2614-9.
Molinar-Toribio E, et al. D-Fagomine attenuates metabolic alterations induced by a high-energy-dense diet in rats. Food Funct. 2015 Aug;6(8):2614-9.

Other Notes

Small volumes of Fagomine, inhibitor vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fagomine purchase

MBS5764546 is an inhibitor targeting Fagomine.