TNF-RII Antibody – Rat Monoclonal

Product Description: The monoclonal antibody HM102 recognizes the extracellular part of membrane-bound TNF-RII as well as the soluble form of TNF-RII which is generated by proteolytic cleavage of the extracellular domain. The soluble form can still bind TNF-alpha with high affinity and functions as a TNF-alpha antagonist. TNF-alpha is an important signalling protein in the immune system which can activate inflammatory responses, induce apoptosis, regulate cellular proliferation, and may even promote cancer progression. TNF-alpha can bind to two structurally distinct membrane receptors, TNF-RI and TNFRII, which have both distinct and overlapping downstream signaling cascades. TNFRI is believed to be expressed on nearly all cell types, whereas TNFRII exhibits more restricted expression, being found on certain subpopulations of immune cells and several other cell types. A dominant role of TNFRII has been shown in thymocyte activation by TNF-alpha, whereas induction of cytotoxicity and other functions are mediated largely by TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is activated only by the membrane bound form of TNF-alpha. The antibody is a agonistic receptor modulating antibody. It enhances in vitro TNF alpha responses by increasing the affinity of the soluble form of TNF-alpha for TNF-RII.

1. Tacchini-Cottier, F et al; Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice. J Immunol 1998, 160: 6182 2. Brekke, O et al. Specificity of endogenous fatty acid release during tumor necrosis factor-induced apoptosis in WEHI164 fibrosarcoma cells. J of Lipid res. 1999, 40:2223
3. Marchetti, L et al; Tumor Necrosis Factor (TNF)-mediated neuroprotection against glutamate-induced excitotoxicity is enhanced by N-methyl-D-aspartate receptor activation. J Biol Chem 2004, 279: 32869
4. Taoufik, E et al; FLIPL protects neurons against in vivo ischemia and in vitro glucose deprivation-induced cell death. J Neurosci 2007, 27: 6633
5. Veroni, C et al; Activation of TNF receptor 2 in microglia promotes induction of anti-inflammatory pathways. Mol Cell Neurosci 2010, 45: 234
6. Chee, J et al. TNF receptor 1 deficiency increases regulatory T cell function in nonobese diabetic mice. J immunol 2011, 187:1702-1712

Small volumes of anti-TNF-RII antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584057 is an antibody targeting TNF-RII. Also known as TNF-RII, Mouse, mAb FITC. Reactive in Mouse. Host: Rat.