C4D Antibody – Mouse Monoclonal

Product Description: The monoclonal antibody 12D11 (also known as clone M4d3) recognizes an epitope on the alpha 2 chain of human C4d. Complement factor C4 (MW 41 kDa), formerly known as Gg protein, consists of an alpha-, beta- and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. Activated C1, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved by factor I resulting in C4c and C4d, in which step all functional sites are lost. The C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. The thioester formed between the side chains of Cys1010 and Gln1013 within the C4d region of the α-chain mediates covalent attachment to the target surface bearing activated forms of C1s or MASP. Furthermore, C4d is highly homologous to C3d with over 35% shared amino acid sequence. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies. The monoclonal antibody 12D11 recognizes C4, C4b and C4d. Antibody 12D11 cross reacts with pig, bovine, baboon and rhesus monkey.

1. Zwirner, J et al; Classical pathway of complement activation in normal and diseased human glomeruli. Kidney Int 1989, 36: 1069. 2. Zwirner, J et al; Classical pathway of complement activation in mammalian kidneys. Immunology 1993, 80: 162.
3. Feucht, H et al; Capillary deposition of C4d complement fragment and early renal graft loss. Kidney Int 1993, 43: 1333.

Small volumes of anti-C4d antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584068 is an antibody targeting C4d. Also known as C4d, Human, mAb 12D11. Host: Mouse.