Nitrotyrosine Antibody – Mouse Monoclonal

Product Description: The monoclonal antibody HM.11 recognizes modified amino acid nitrotyrosine in all different species. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage. The monoclonal antibody HM.11 recognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine

1. ter Steege, JCA et al; Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA. Free Radic Biol Med 1998, 25: 953 2. Casoni, F et al; Protein nitration in a mouse model of familial amyotrophic lateral sclerosis. J Biol Chem 2005, 280: 16295
3. Kooy, NW et al; Evidence for in vivo peroxynitrite production in human acute lung injury. Am J Respir Crit Care Med 1995, 151: 1250 v 4. Klausz, G et al; Local and peripheral cytokine response and CagA status of Helicobacter pylori-positive patients with duodenal ulcer. Eur Cytokine Netw 2003, 14: 143

Small volumes of anti-Nitrotyrosine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584095 is an antibody targeting Nitrotyrosine. Also known as Nitrotyrosine, mAb 100 ìg 267. Host: Mouse.