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PAI-1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS584097

Product Description: Plasminogen activator inhibitor type-1 (PAI-1), a member of the serine protease inhibitor (serpin) superfamily, is an important protein in the regulation of fibrinolysis. PAI-1 is unique among the serpins because of its functional and… More details

$750 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
PAI-1, Monoclonal Antibody
Full Product Name
PAI-1, Human, mAb MA-33H1F7
Product Gene Name
anti-PAI-1 antibody
Product Synonym Names
PAI-1, endothelial plasminogen activator inhibitor, serpin E1
Clonality
Monoclonal
Isotype
Mouse IgG1
Host
Mouse
Species Reactivity
Reactivity: Human. Cross reactivity: Mouse, Rabbit, Rat
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Molecular Weight
43,404 Da
OMIM
gene 613329
Immunogen
Human PAI-1/t-PA complex
Applications Tested/Suitable for anti-PAI-1 antibody
FS, Immunoassay (IA), Western Blot (WB)
Application Notes for anti-PAI-1 antibody
Application Notes: W: A non-reduced sample treatment and SDS-Page was used. The band size is 52 kDa (Ref.5).
Application Use: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user's experimental setting.

Product References and Citations for anti-PAI-1 antibody

1. Debrock, S et al; Neutralization of plasminogen activator inhibitor-1 inhibitory properties: identification of two different mechanisms. Biochim Biophys Acta 1997, 1337: 257 2. Berry, C et al; Antithrombotic activity of a monoclonal antibody inducing the substrate form of plasminogen activator inhibitor type 1 in rat models of venous and arterial thrombosis. Br J Pharm 1998, 125: 29
3. Bijnens, A et al; Importance of the hinge region between alpha-helix F and the main part of serpins, based upon identification of the epitope of plasminogen activator inhibitor type 1 neutralizing antibodies. J Biol Chem 2000, 275: 6375
4. Rupin, A et al; Inactivation of plasminogen activator inhibitor-1 accelerates thrombolysis of a platelet-rich thrombus in rat mesenteric arterioles. Thromb Haemst 2001, 86: 1528
5. Sironi, L et al; Effect of valsartan on angiotensin ll-induced plasminogen activator inhibitor-1 biosynthesis in arterial smooth muscle cells. J Am Heart Ass 2001, 37: 961
6. Komissarov, A et al; Redirection of the reaction between activated protein C and a serpin to the substrate pathway. Thromb Res. 2008, 122: 397

Other Notes

Small volumes of anti-PAI-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAI-1 purchase

MBS584097 is an antibody targeting PAI-1. Also known as PAI-1, Human, mAb MA-33H1F7. Reactive in Reactivity: Human. Cross reactivity: Mouse, Rabbit, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PAI-1. It may not necessarily be applicable to this product.

NCBI GI #
10835159
NCBI GeneID
5054
NCBI Accession #
NP_000593.1
UniProt Secondary Accession #
B7Z4S0; F8WD53
UniProt Related Accession #
P05121
GenBank Nucleotide #
NM_000602.4
NCBI Official Full Name
plasminogen activator inhibitor 1
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
NCBI Official Symbol
SERPINE1
NCBI Official Synonym Symbols
PAI; PAI1; PAI-1; PLANH1
UniProt Protein Name
Plasminogen activator inhibitor 1
UniProt Synonym Protein Names
Endothelial plasminogen activator inhibitor; Serpin E1
Protein Family
Plasminogen activator inhibitor
UniProt Gene Name
SERPINE1
UniProt Synonym Gene Names
PAI1; PLANH1; PAI; PAI-1
UniProt Entry Name
PAI1_HUMAN
NCBI Protein Information
plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

NCBI Summary for PAI-1

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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UniProt Comments for PAI-1

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; regulation of receptor activity; angiogenesis; chronological cell aging; negative regulation of cell migration; negative regulation of cell adhesion mediated by integrin; transcription initiation from RNA polymerase II promoter; platelet activation; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; fibrinolysis; positive regulation of angiogenesis; positive regulation of transcription from RNA polymerase II promoter; gene expression; blood coagulation; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency
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Research Articles on PAI-1

1. A compound of Astragalus and Salvia suppresses drug-induced hepatocarcinogenesis by inhibiting fibrosis and PAI-1 mRNA transcription.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAI-1 antibody

 Reference Product  PubMed Publications
 TGFB1 antibody  >517 publications with PAI-1 and TGFB1
 SMAD4 antibody  >82 publications with PAI-1 and SMAD4
 HIF1A antibody  >63 publications with PAI-1 and HIF1A
 EGF antibody  >61 publications with PAI-1 and EGF
 PLG antibody  >43 publications with PAI-1 and PLG
 PLAT antibody  >23 publications with PAI-1 and PLAT
 PLAU antibody  >12 publications with PAI-1 and PLAU
 VTN antibody  >5 publications with PAI-1 and VTN
 ALB antibody  >3 publications with PAI-1 and ALB

Pathways associated with anti-PAI-1 antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies  BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram

Diseases associated with anti-PAI-1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3155 publications with PAI-1 and Cardiovascular Diseases
 Thrombosis Antibodies  >1377 publications with PAI-1 and Thrombosis
 Inflammation Antibodies  >1286 publications with PAI-1 and Inflammation
 Heart Diseases Antibodies  >1035 publications with PAI-1 and Heart Diseases
 Necrosis Antibodies  >862 publications with PAI-1 and Necrosis
 Atherosclerosis Antibodies  >766 publications with PAI-1 and Atherosclerosis
 Hypertension Antibodies  >691 publications with PAI-1 and Hypertension
 Fibrosis Antibodies  >671 publications with PAI-1 and Fibrosis
 Kidney Diseases Antibodies  >519 publications with PAI-1 and Kidney Diseases
 Nervous System Diseases Antibodies  >515 publications with PAI-1 and Nervous System Diseases
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