C5aR Antibody – Rat Monoclonal

Product Description: The monoclonal antibody 20/70 recognizes the mouse receptor for C5a (CD88). The mouse anaphylatoxin complement factor C5a, a split product of C5, is one of the most potent inflammatory chemoattractants and interacts with two C51 receptors, C5aR and C5L2.- Most of the C5a effects are mediated through C5aR. C5aR is known to be crucial in the initiation of acute inflammatory responses. C5a receptor is a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 45 kDa C5a receptor is expressed by leukocytes such as neutrophils, eosinophils, basophils, monocytes, dendritic cells and mast cells. C5aR can be expressed on both immune and nonimmune cells. Nonimmune cells expressing C5aR include vascular endothelial cells, cardiomyocytes and bronchial epithelial cells. Signaling of C5aR involves intracellular calcium mobilization and activation of among others protein kinase- B (PKB) and protein phospholipase D (PLD) pathways. High C5aR expression levels have been associated with the pathogenesis of many inflammatory conditions, autoimmune and neurodegenerative diseases like rheumatoid arthritis, multiple sclerosis, atherosclerosis and inflammatory bowel diseases. In vivo blockade of C5aR greatly reduces inflammatory injury. The monoclonal antibody 20/70 has been used in functional studies and is capable- to prevent the binding of C5a to its receptor.

1. Shushakova, N et al; C5a anaphylatoxin is a major regulator of activating versus inhibitory FcγRs in immune complex-induced lung disease. J Clin Invest 2002, 110: 1823 2. Soruri, A et al; Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody. Immunol Lett 2003, 88: 47
3. Godau, J et al; C5a initiates the inflammatory cascade in immune complex peritonitis. J Immunol 2004, 173: 3437
4. Chen, NJ et al; C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a. Nature 2007, 446: 203
5. Rittirsch, D et al; Functional roles for C5a receptors in sepsis. Nat Med 2008, 14: 551
6. Gueler, F et al; Complement 5a Receptor inhibition improves renal allograft survival. J Am Soc Nephrol 2008, 19: 2302

Small volumes of anti-C5aR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584113 is an antibody targeting C5aR. Also known as C5aR, Mouse, mAb 20/70. Reactive in Mouse. Host: Rat.