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TNF-RII Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS584119

Product Description: The antibody MR2-1 reacts with the extra-cellular part of the TNF-RII. It also reacts with the soluble receptor. TNF-RII is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-RII… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TNF-RII, Monoclonal Antibody
Full Product Name
TNF-RII, Human, mAb MR2-1
Product Gene Name
anti-TNF-RII antibody
Product Synonym Names
Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNFR-II, p75, p80 TNF-alpha receptor, CD120b
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
MR2-1
Host
Mouse
Form/Format
0.2 µm filtered in PBS + 0.1% BSA
Purity/Purification
Protein G
Concentration
100 µg/ml (lot specific)
Preparation and Storage
Store at 4°C
Cross reactivity
Cynomolgus monkey, Rhesus monkey
Species
Human
Endotoxin
17.37 EU/mg
Immunogen
sTNF-R75 purified from the culture supernatant of NSO cells (transfected with the extracellular part of TNF-R75)
Applications Tested/Suitable for anti-TNF-RII antibody
Immunofluorescence (IF), Flow Cytometry (FC), Functional Studies (FS), Immuno Assay (IA), Immunoprecipitaion (IP), Western Blot (WB)
Application Notes for anti-TNF-RII antibody
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.


IA: MBS584119 can be used as a capture antibody.
IP: The lysate was centrifuged and the supernatant was incubated with 5 mg TNFR2-specific antibody MR2-1 for 2 hours at 4°C. The immuno complexes were precipitated with protein G agarose for two hours at 4°C.
W: A reduced protocol was used. The expected band size is ~65 kDa.
FS: The monoclonal antibody MR2-1 is an agonistic antibody (after cross-linking) and receptor modulating antibody useful for cell culture experiments. The reactivity of the antibody MR2-1 with cell-bound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha.
Positive control: human lymphnodes for frozen sections or PHA activated T cells for flow cytometry.

Flow cytometry of anti-TNF-RII antibody

Flow cytometry with THP-1 cells. The black line represents cells only, the red line the isotype control and the blue line (MBS584119) (1 µg/250000 cells)
Mouse Anti-TNF-RII Monoclonal Antibody – Cat# MBS584119

ImmunoAssay (IA) of anti-TNF-RII antibody

Immuno Assay experiment with (MBS584119) as capture antibody used in different concentrations.
Mouse Anti-TNF-RII Monoclonal Antibody, ImmunoAssay (IA) – Cat# MBS584119

Product References and Citations for anti-TNF-RII antibody

1. Leeuwenberg, JFM et al; Slow release of soluble TNF-Receptors by monocytes in vitro. J Immunol 1994, 152: 4036 2. Leeuwenberg, JFM et al; Lipopolysaccharide LPS-mediated soluble TNF-Receptor release and TNF-Receptor expression by monocytes; role of CD14, LPS binding protein and bactericidal/permeability-increasing protein. J Immnol 1994, 152: 5070
3. Marchetti, L et al; Tumor necrosis factor (TNF)-mediated neuroprotection against glutamate-induced excitotoxicity is enhanced by N-methyl-D-aspartate receptor activation. Essential role of a TNF receptor 2-mediated phosphatidylinositol 3-kinase-dependent NF-kappa B pathway. J Biol Chem 2004, 279: 32869

Other Notes

Small volumes of anti-TNF-RII antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNF-RII purchase

MBS584119 is an antibody targeting TNF-RII. Also known as TNF-RII, Human, mAb MR2-1. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TNF-RII. It may not necessarily be applicable to this product.

NCBI GI #
315423985
NCBI GeneID
338033
NCBI Accession #
ADU17359.1
NCBI Official Full Name
tumor necrosis factor receptor type two, partial
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 1B
NCBI Official Symbol
TNFRSF1B
NCBI Official Synonym Symbols
TNF-RII/TNFRSF1B
UniProt Protein Name
Tumor necrosis factor receptor type two
UniProt Synonym Protein Names
Tumor necrosis factor receptor type two
Protein Family
Tumor necrosis factor receptor superfamily
UniProt Gene Name
TNF-RII/TNFRSF1B
UniProt Entry Name
E7CZZ7_BOVIN
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1B

Research Articles on TNF-RII

1. These results suggest that TNF-alpha sources include immune cells, as well as large and small luteal cells, and that TNF-RI and TNF-RII are present in the luteal cells of the bovine corpus luteum.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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