TNF-RI Antibody – Mouse Monoclonal

Product Description: The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.

1. Thoma, B et al; Identification of a 60 kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. J Exp Med 1990, 172: 1019 2. Grell, M et al; TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis. Lymphokine Cytokine Res 1993, 12: 143
3. Scheurich, P et al; Agonistic and antagonistic antibodies as a tool to study the functional role of human tumor necrosis factor receptors. Tumor Necrosis factor 1993, 4: 52
4. Grell, M et al; The type 1 receptor (CD120a) is the high-affinity receptor for soluble tumor necrosis factor. Proc Natl Acad Sci USA 1998, 95: 570
5. Krippner-Heidenreich, A et al; Single-chain TNF, a TNF derivative with enhanced stability and antitumoral activity. J Immunol 2008, 180: 8176
6. Kontermann, R et al; A humanized tumor necrosis factor receptor 1 (TNFR1)-specific antagonistic Antibody for selective inhibition of tumor necrosis factor (TNF) action. J Immunother 2008, 31: 225

Small volumes of anti-TNF-RI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584218 is an antibody targeting TNF-RI. Also known as TNF-RI, Human, mAb H398, FITC. Reactive in Reactivity: Human. Cross reactivity: Rat. Host: Mouse.