Macrophages F4/80 Antibody – Rat Monoclonal

Product Description: The monoclonal antibody BM8 recognises a 125 kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. The monoclonal antibody BM8 cross reacts with human heart macrophages.

1. Malorny, U et al; A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res 1986, 243: 421 2. Leenen, P et al; Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 1994, 174: 5
3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879
4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035

Small volumes of anti-Macrophages F4/80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584231 is an antibody targeting Macrophages F4/80. Also known as Macrophages F4/80, Mouse, mAb BM8, FITC. Reactive in Mouse. Host: Rat.