SIGN-R1 Antibody – Rat Monoclonal

Product Description: The monoclonal antibody ER-TR9 recognizes murine SIGN-related 1 (SIGN-R1). Mouse SIGN-R1,- a homolog of human DC-SIGN, is a 37 kDa type II transmembrane protein containing a single, C-terminal C-type lectin domain. SIGN-R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in the peritoneal cavity of some mouse strains. ER-TR9 does not react with macrophages in other regions of the spleen, such as CD169+ marginal metallophils and F4/80+ red pulp macrophages. In the spleen, the MZM function in trapping and clearance of blood-borne microbial antigens. SIGN-R1 mediates the uptake of encapsulated microbes, particularly through the recognition of microbial polysaccharides. Uptake of FITC-labeled dextran by macrophages can be blocked both in vivo and in vitro by the monoclonal antibody ER-TR9. Therefore, the monoclonal antibody ER-TR9 can be used to study the uptake of polysaccharides by macrophages.

1. Dijkstra, C, et al; Marginal zone macrophages identified by a monoclonal antibody: characterization of immune- and enzyme-histochemical properties and functional capacities. Immunology 1985, 55: 23 2. Kretschmer, K, et al; The selection of marginal zone B cells differs from that of B-1a cells. J Immunol 2003, 171: 6495
3. Kang, Y et al; The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. PNAS 2004, 101: 215
4. Taylor, P et al; The role of SIGN-R1 and the beta-glucan receptor (dectin-1) in the nonopsonic recognition of yeast by specific macrophages. J Immunol 2004, 172: 1157
5. Nagaoka, K et al; Association of SIGNR1 with TLR4-MD-2 enhances signal transduction by recognition of LPS in gram-negative bacteria. Int Immunol 2005, 17: 827
6. Takagi, H et al. Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages. Glycobiology 2009, 19:258

Small volumes of anti-SIGN-R1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584242 is an antibody targeting SIGN-R1. Also known as SIGN-R1, Mouse, mAb ER-TR9. Reactive in Mouse. Host: Rat.