Rat Anti-PECAM-1 Monoclonal Antibody – Cat# MBS584249

PECAM-1 Antibody – Rat Monoclonal

Name: PECAM-1 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS584249
Price: 730 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS584249

Product Description: The monoclonal antibody MEC7.46 recognizes the mouse form of the platelet-endothelial cell adhesion molecule (PECAM)-1 (CD31). PECAM-1 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PECAM-1, Monoclonal Antibody

Full Product Name

PECAM-1, Mouse, mAb MEC7.46, FITC

Product Gene Name

anti-PECAM-1 antibody

Product Synonym Names

CD31, platelet-endothelial cell adhesion molecule-1

Clonality

Monoclonal

Isotype

Rat IgG1

Host

Rat

Species Reactivity

Mouse

Form/Format

1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.

Preparation and Storage

Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Molecular Weight

81,378 Da

OMIM

173445

Conjugate

FITC

Immunogen

Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)

Positive Control

Mouse t-end.1 cells

Applications Tested/Suitable for anti-PECAM-1 antibody

F, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Application Notes for anti-PECAM-1 antibody

Application Notes: FC: Antibody MEC7.46 stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1)
Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product References and Citations for anti-PECAM-1 antibody

1. Vecchi, A et al; Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium. Eur J Cell Biol 1994, 63: 247 2. Xu, Q et al; Circulating progenitor cells regenerate endothelium of vein graft atherosclerosis, which is diminished in ApoE-deficient mice. Circ Res 2003, 93: e76
3. Smetsers, T et al; Localization and characterization of melanoma-associated glycosaminoglycans: Differential expression of chondroitin and heparan sulfate epitopes in melanoma. Canc Res 2003, 63: 2965

Other Notes

Small volumes of anti-PECAM-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PECAM-1 purchase

MBS584249 is an antibody targeting PECAM-1. Also known as PECAM-1, Mouse, mAb MEC7.46, FITC. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for PECAM-1. It may not necessarily be applicable to this product.

NCBI GI #

189776

NCBI GeneID

5175

NCBI Accession #

AAA36429.1

UniProt Secondary Accession #

Q6LDA9; Q8TBH1; Q96RF5; Q96RF6; Q9NP65; Q9NPB7; Q9NPG9; Q9NQS9; Q9NQT0; A8K3S7; D3DU31

UniProt Related Accession #

P16284

NCBI Official Full Name

PECAM-1

NCBI Official Synonym Full Names

platelet/endothelial cell adhesion molecule 1

NCBI Official Symbol

PECAM1

NCBI Official Synonym Symbols

CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

UniProt Protein Name

Platelet endothelial cell adhesion molecule

UniProt Synonym Protein Names

EndoCAM; GPIIA'; PECA1; CD_antigen: CD31

Protein Family

Platelet endothelial cell adhesion molecule

UniProt Gene Name

PECAM1

UniProt Synonym Gene Names

PECAM-1

UniProt Entry Name

PECA1_HUMAN

NCBI Protein Information

platelet endothelial cell adhesion molecule; CD31 antigen

NCBI Summary for PECAM-1

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

UniProt Comments for PECAM-1

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; signal transduction; cell adhesion; blood coagulation; leukocyte migration; phagocytosis

Research Articles on PECAM-1

1. results of the current meta-analysis suggest that homozygous polymorphic genotype (GG) of the rs1131012 SNP may confer protection against myocardial infarction

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PECAM-1 antibody

Products by Pathway	Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram

Diseases associated with anti-PECAM-1 antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>614 publications with PECAM-1 and Cardiovascular Diseases
Inflammation Antibodies	>485 publications with PECAM-1 and Inflammation
Necrosis Antibodies	>399 publications with PECAM-1 and Necrosis
Neoplasms, Experimental Antibodies	>258 publications with PECAM-1 and Neoplasms, Experimental
Lung Diseases Antibodies	>234 publications with PECAM-1 and Lung Diseases
Heart Diseases Antibodies	>228 publications with PECAM-1 and Heart Diseases
Breast Neoplasms Antibodies	>204 publications with PECAM-1 and Breast Neoplasms
Lung Neoplasms Antibodies	>183 publications with PECAM-1 and Lung Neoplasms
Liver Diseases Antibodies	>171 publications with PECAM-1 and Liver Diseases
Atherosclerosis Antibodies	>144 publications with PECAM-1 and Atherosclerosis