Fibroblasts Antibody – Rat Monoclonal

Product Description: Monoclonal antibody ER-TR7 recognizes with an intracellular component of mouse fibroblasts. Fibroblasts are the least specialized cells in the connective-tissue family. They are dispersed in connective tissue throughout the body, where they secrete a nonrigid extracellular matrix (ECM) that is rich in type I and/or type III collagen. Conective tissue consists of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. The activation of fibroblasts by inflammatory stimuli results in their migration, proliferation and deposition of extracellular matrix components, important features involved in both wound healing and fibrosis.
The ER-TR7 antigen is a ubiquitous component of stromal (interstitial) matrix cartilage and of at least some basement membrane zones. The antigen detected is not a basement membrane component, nor any major collagen type or fibronectin. The antigen detected has a wider tissue distribution than reticulin. ER-TR7 detects an intracellular component of fibroblasts. Since ER-TR7 does not react with purified laminin, collagen types I-V, fibronectin, heparin sulfate proteoglycan, entactin or nidogen, it detects a hitherto uncharacterized antigen.
The monoclonal antibody ER-TR7 can be used to study the micro-anatomy of various organs. ER-TR7 outlines the various compartments of peripheral lymphoid organs by characteristic labeling patterns (no such compartments are found in central lymphoid organs). Furthermore ER-TR7 delineates various types of connective tissue compartments in nonlymphoid organs.
The antibody ER-TR7 detects reticular fibroblasts, which constitute the cellular framework of lymphoid and nonlymphoid organs and their products. ER-TR7 is useful to clearly delineated the follicles, periarteriolar lymphoid sheath and marginal zone; the major white pulp compartments. Furthermore in lymph nodes, the capsule, sinuses, follicles, paracortex and medullary cords are clearly delineated.

1. Vliet van, E et al; Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol 1984, 14: 524 2. Vliet van, E et al; Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody. J Histochem Cytochem 1986, 34: 883
3. Kalled, S et al; Anti-CD40 ligand antibody treatment of SNF 1 mice with established nephritits: preservation of kidney function. J Immunol 1998, 120: 2158
4. Nolte, M et al; A conduit system distributes chemokines and small blood-borne molecules through the splenic white pulp. J Exp Med 2003, 3: 505
5. Svensson, M et al; Stromal cells direct local differentiation of regulatory dendritic cells. Immunity 2004, 21: 505
6. Alba, R et al; Biodistribution and retargeting of FX-binding ablated adenovirus serotype 5 vectors. Blood 2010 (Epub july 7).

Small volumes of anti-Fibroblasts antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584267 is an antibody targeting Fibroblasts. Also known as Fibroblasts, Mouse, mAb ER-TR7. Reactive in Rat. Host: Rat.