C3/C3A Antibody – Mouse Monoclonal

Product Description: Monoclonal antibody 474 reacts with an epitope on C3a. The antibody reacts both with intact C3 as with C3a. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. During complement activation, C3 is proteolytically cleaved resulting in release of the anaphylatoxic peptide C3a. C3a is a small polypeptide consisting of 74 amino acids (ca.10kDa). C3a itself is very short-lived and in serum cleaved rapidly into the more stable C3a-desArg (also called acylation stimulating protein, ASP). C3a exerts its function through a specific receptor (C3aR), which belongs to the G-protein coupled receptor family. Expression of C3aR- has been reported in many cell types including myeloid and non-myeloid cells. Expression of C3aR on haematopoietic stem/progenitor cells has been shown to promote the trafficking/homing of these cells to the BM. Engagement of C3aR on DCs and T cells has been shown to up-regulate these cell functions.
C3a is a mediator of local inflammatory processes. It induces smooth muscle contraction, increases vascular permeability, and causes histamine release from mast cells and basophilic leukocytes. C3a is involved in inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and a variety of autoimmune diseases. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported.

1. Hartmann, H et al; Rapid quantification of C3a and C5a using a combination of chromatographic and immunoassay procedures. J Immunol Methods 1993, 166: 35
2. St¶ve, S et al; Re-evaluation of the storage conditions for blood samples which are used for determination of complement activation. J Immunol Methods 1995, 182: 1
3. Li, R et al. Localized-statistical quantification of human serum proteome associated with type 2 diabetes. Plos one 2008, 3:e3224
4. Shi, L et al. Discovery and identification of potential biomarkers of pediatric acute lymphoblastic leukemia. Proteome science 2009, 7:7

Small volumes of anti-C3/C3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584407 is an antibody targeting C3/C3a. Also known as C3/C3a, Human, mAb 474. Reactive in Human. Host: Mouse.