Activated C3 Antibody – Mouse Monoclonal

Product Description: The monoclonal antibody bH6 recognizes a neo-epitope expressed on the cleavage fragments of C3b, iC3b, and C3c. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. Monoclonal antibodies detecting neo-epitopes have been used for direct quantification of activation at different steps in the complement cascade. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. The monoclonal antibody bH6 is specific for a C3 neo-epitope expressed on the cleavage fragments of C3b, iC3b, and C3c, but not C3dg and C3f.

1. Garred, P et al; Characterization of a monoclonal antibody MoAb bH6 reacting with a neo-epitope of human C3 expressed on C3b, iC3b, and C3c. Scand J Immunol 1988, 27: 319
2. Halstensen T et al; Epithelial deposition of immunoglobulin G1 and activated complement (C3b and terminal complement complex) in ulcerative colitis. Gastroenterology 1990, 98: 1264
3. Garred P et al; Quantification in enzyme-linked immunosorbent assay of a C3 neo-epitope expressed on activated human complement factor C3. Scand J Immunol 1988, 27: 329
4. Lappeg¥rd, K et al; Effect of complement inhibition and heparin coating on artificial surface - induced leukocyte and platelet activation. Ann Thorac Surg 2004, 77: 932

Small volumes of anti-Activated C3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584410 is an antibody targeting Activated C3. Also known as Activated C3, Human, mAb bH6. Reactive in Human. Host: Mouse.