C5aR Antibody – Mouse Monoclonal

Product Description: The monoclonal antibody S5/1 recognizes the human receptor for C5a (CD88). The C5a receptor- is a seven transmembrane GTP-binding-protein-coupled receptor of 45kDa which binds with high affinity to human anaphylatoxin C5a. Upon C5a binding- G-protein-dependent cellular- responses are initiated such as increased intracellular Ca2+, granule fusion with the cell membrane, enzyme release and oxidative burst suggesting that C5aR is an important mediator of inflammation. The C5a receptor is expressed on a broad range of cell types, including- neutrophils, monocytes/macrophages, dendritic cells, astrocytes and microglia.
Interestingly, C5a receptor signalling has been associated with several inflammatory diseases including rheumatoid arthritis.-
Clone S5/1 was raised against a synthetic peptide comprising the N-terminal domain of the C5aR (Met1-Asn31). The antibody has been shown to inhibit the binding of C5a to its receptor.

1. Opperman, M et al; Probing the human receptor for C5a anaphylatoxin with side-directed antibodies. J Immunol 1993, 151: 3785 2. Opperman, M et al; Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors. Immunol. 1994, 82: 516
3. Werfel, T et al; C5a receptors are detectable on mast cells in normal human skin and in psoriatic plaques but not in weal and flare reactions or in urticaria pigmentosa by immunohistochemistry. Arch Dermatol Res 1997, 289: 83
4. Zwirner, J et al; Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies. Immunology 1999, 97: 166
5. Fortin, J et al; Contractile effect of anaphylatoxin C5a and of a mimetic peptide on the human umbilical artery: further evidence for leukocyte-dependent vasomotion. J Cardiovasc Pharmacol. 2002, 40:815
6. Gueler, F et al; Complement 5a receptor inhibition improves renal allograft survival. J Am Soc Nephrol 2008, 19: 2302
7. Strey, CW et al;. Complement and neutrophil function changes after liver resection in humans. World J Surg 2009, 33: 2635
8. Werfel, T et al: CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin. J Immunol 1996, 157: 1729.
9. Petitclerc, E et al: Pathologic leukocyte infiltration of the rabbit aorta confers a vasomotor effect to chemotactic peptides through cyclooxygenase-derived metabolites. J Immunol 1996, 156: 3426

Small volumes of anti-C5aR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584430 is an antibody targeting C5aR. Also known as C5aR, Human, mAb S5/1. Reactive in Reactivity: Human. Cross reactivity: Rabbit. Host: Mouse.