Azurocidin (AZU1) ELISA Kit for Human

Intended Uses: The human Azurocidin ELISA kit is to be used for the in vitro quantitative determination of human azurocidin in plasma, serum and sputum samples. This kit is intended for laboratory research use only. The analysis should be performed by trained laboratory professionals.

Background/Introduction: Azurocidin, also called heparin-binding protein (HBP) or cationic antimicrobial protein of 37 Kda (CAP37), is an inactive homolog of serine proteinases residing in granulocytes. Initially it was thought to have only activity against gram-negative bacteria. Later it was also found to act against gram-positive bacteria and C.albicans. Azurocidin is considered a family member of polymorphonuclear leukocytes- derived antimicrobial proteins like defensins, LL-37 and lysozyme. Azurocidin has been recognized as a player in the activation and modulator of the immune response and may act to alarm the immune system. The cationic domain at one side of the protein is essential for its antimicrobial activity. It is stored in azurophil granules as well as secretory granules and as a result partly released at early stage of extravasation. Azurocidin is involved in chemotaxis and activation of monocytes, cytokine release and phagocytosis leading to more efficient bacterial clearance. The primary targeted environment of azurocidin are cells in the bloodstream, that is the endothelial lining, and the extravascular surroundings. The interaction of azurocidin with leucocytes is mediated via beta2-intergrins.

Small volumes of AZU1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584838 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Azurocidin (AZU1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AZU1. The ELISA analytical biochemical technique of the MBS584838 kit is based on AZU1 antibody-AZU1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AZU1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AZU1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).