TLR4 Antibody – Mouse Monoclonal

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. The HTA125 monoclonal antibody reacts preferentially, especially in flow cytometry, with human TLR4 (CD284) that is associated with MD-2. HTA125 is a TLR4 (CD284) function-blocking antibody that is useful for studies on the role of TLR4 (CD284) as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to precipitate TLR4 (100 kDa). The antibody HTA125 is cross reactive with canine, cynomolgus monkey, rhesus monkey and marmoset monkey.

1. Shimazu, R et al; MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med 1999, 189: 1777
2. Tabeta, K et al; Toll-like receptors confer responsiveness to lipopolysaccharide from porphyromonas gingivalis inHuman gingival fibroblasts. Infect Immun 2000, 68: 3731
3. Akashi, S et al; Regulatory roles for CD14 and phosphatidylinositol in the signaling via Toll-like receptor 4-MD-2. Biochem Biophys Res Commun 2000, 268: 172
4. Wang, J et al; Involvement of CD14 and Toll-like receptors in activation ofHuman monocytes by Aspergillus fumigatus hyphae. Infect Immun 2001, 69: 24025. Walton, K et al; Receptors involved in the oxidized 1-palmitoyl-2 arachidonoyl-sn-glycero-3- phosphorylcholine-mediated synthesis of interleukin-8. J Biol Chem 2003, 278: 296616. Triantafilou, M et al; Lateral diffusion of Toll-like receptors reveals that they are transiently confined within lipid rafts on the plasma membrane. J Cell Sci 2004, 117: 4007
7. Elner, S et al; TLR4 mediatesHuman retinal pigment epithelial endotoxin binding and cytokine expression. Trans Am Ophthalmol Soc 2005, 103: 126
8. Burgener, I et al; Antibodies specific forHuman or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124: 184
9. Brüll, F et al; TLR2 activation is essential to induce a Th1 shift inHuman peripheral blood mononuclear cells by plant stanols and plant sterols. J Biol Chem 2010, 285: 2951

Small volumes of anti-TLR4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584871 is an antibody targeting TLR4. Also known as TLR4, Human, mAb HTA125, biotinylated. Reactive in Human, Canine, Monkey. Host: Mouse.