Sthrombomodulin ELISA Kit for Human

Background: Thrombomodulin (TM; CD141 or BDCA-3) is a 557 amino acid transmembrane glycoprotein predominantly expressed on the luminal surface of endothelial cells of most blood vessels, but also present in other gestational tissues like placenta and myometrium, polymorphonuclear neutrophils and monocytes. TM plays a central role in protection against excessive coagulation via protein C pathway, with subsequent inactivation of coagulation V and VIII. After processing and removal of the N-terminal site a 70-100KDa protein is obtained. TM consist of 5 domains: D1. CTLD, EGF-like D2, extracellular serine/threonine D3, transmembrane D4 and cytoplasmic D5. TM forms a complex with thrombin. This complex is needed in the conversion of protein C into its activated form (APC). APC leads to degradation of coagulation factor Va and VIIIa leading to continuation of its downstream pathway and consumption of thrombin. The binding to thrombin is 1:1 and the complex accelerated the activation of protein C significantly. When the coagulation cascade is activated, prothrombin is converted to thrombin by coagulation factors Va and VIIIa, ultimately leading to fibrin clot formation. Although its primary function is to mediate endothelial thromboresistance, soluble variants comprising the extracellular domains are found in eg. serum and urine. Soluble TM (sTM) is considered as a marker of endothelial damage and retains its anticoagulant characteristics. Increased levels of sTM have been associated with inflammatory diseases related to damage endothelium like atherosclerosis and lung injury. STM has also been linked to inflammatory conditions. TM/thrombin complex can suppress cytokine production via APC. TM is also effective in the complement system by accelerating factor I inactivation of C3b. Mutations in TM can lead to overactive complement possibly leading to atypical hemolytic uremic syndrome. Thrombomodulin assay HK383 is a 4.5h ELISA- assay making use of two monoclonal antibodies and uses a recombinant protein as standard. Parallelism between this standard and native human thrombomodulin has been shown, validating the use of this assay to reliably quantify human TM is samples.

Intended Uses: The Human soluble Thrombomodulin (sThrombomodulin) ELISA kit is to be used for the in vitro quantitative determination of sThrombomodulin in serum and plasma samples. This kit is intended for laboratory research use only and is not for use in test or therapeutic procedures. The analysis should be performed by trained laboratory professionals.

Small volumes of sThrombomodulin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584884 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sThrombomodulin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing sThrombomodulin. The ELISA analytical biochemical technique of the MBS584884 kit is based on sThrombomodulin antibody-sThrombomodulin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect sThrombomodulin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, sThrombomodulin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).