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Collagen XVII (COL17A1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS588018

Collagen XVII is a member of the transmembrane collagen family of proteins. It is a major structural component of the hemidesmosome (HD) playing an important role in its integrity. HD is a specialized multiprotein complex involved in the attachment of basal… More details

$630 / 0.25 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Collagen XVII (COL17A1), Monoclonal Antibody
Full Product Name
Collagen XVII Mouse Monoclonal Antibody (NC16a-3)
Product Gene Name
Product Synonym Names
Collagen alpha-1 (XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; BP180; BPAG2
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NC16a-3
Host
Mouse
Form/Format
Purified with protein G, stored in PBS pH 7.4 and lyophilized.
Specificity
The antibody recognizes the NC16a domain (aa545-557)
Preparation and Storage
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
Preservative
None
Target
Collagen XVII
Target Alias
Collagen alpha-1(XVII) chain, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, COL17A1, BP180, BPAG2
Immunogen
Purified recombinant NC16a-domain of collagen XVII
Applications Tested/Suitable for anti-COL17A1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-COL17A1 antibody
If reconstituted with deionized water in 250 uL: WB 1:1000; IHC-P: 1:40. Optimal dilution has to be determined by the user

Product References and Citations for anti-COL17A1 antibody

1. Hofmann SC-Plasmin plays a role in the in vitro generation of the linear IgA dermatosis antigen LADB97. 2. Seppanen A-Distribution of collagen XVII in the human brain. 3. Seppanen A-Expression of collagen XVII and ubiquitin-binding protein p62 in motor neuron disease.

Other Notes

Small volumes of anti-COL17A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COL17A1 purchase

MBS588018 is an antibody targeting COL17A1. Also known as Collagen XVII Mouse Monoclonal Antibody (NC16a-3). Host: Mouse.
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