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Estrogen Sulfotransferase (EST) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS588287

Estrogen sulfotransferase (EST) is a cytosolic enzyme that catalyzes specific sulfonation with a high affinity for estrogens. Because EST is involved in estrone and estradiol metabolism, its expression in the male reproductive tract determines where, along… More details

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Product Overview

Product Name
Estrogen Sulfotransferase (EST), Polyclonal Antibody
Full Product Name
Estrogen Sulfotransferase (EST) Rabbit Polyclonal Antibody
Product Gene Name
Product Synonym Names
anti-estrogen sulfotransferase; anti-est; Sulfotransferase 1E1; ST1E1; Sulfotransferase; estrogen-preferring; SULT1E1
Clonality
Polyclonal
Host
Rabbit
Form/Format
Lyophilized immunogen affinity purified in PBS pH7.4
Specificity
A highly specific and sensitive antibody against the bovine Estrogen Sulfotransferase.
Preparation and Storage
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (1 year). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (1 year lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay
3D Structure
ModBase 3D Structure for P49888
Immunogen
Full length recombinant bovine EST protein
Target
Estrogen Sulfotransferase (EST)
Applications Tested/Suitable for anti-EST antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-EST antibody
If reconstituted in 100muL: WB: 1 mug/ml; IHC 5 mug/ml; IF 10 mug/ml

Product References and Citations for anti-EST antibody

1. Blaschka C-Occurrence of sulfonated steroids and ovarian expression of steroid sulfatase and SULT1E1 in cyclic cows. 2. Frenette G-Estrogen sulfotransferase is highly expressed along the bovine epididymis and is secreted into the intraluminal environment.

Other Notes

Small volumes of anti-EST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EST purchase

MBS588287 is an antibody targeting EST. Also known as Estrogen Sulfotransferase (EST) Rabbit Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EST. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
estrogen sulfotransferase
NCBI Official Synonym Full Names
sulfotransferase family 1E member 1
NCBI Official Symbol
SULT1E1
NCBI Official Synonym Symbols
EST; STE; EST-1; ST1E1
UniProt Protein Name
Estrogen sulfotransferase
UniProt Synonym Protein Names
EST-1; Sulfotransferase 1E1; ST1E1; Sulfotransferase, estrogen-preferring
Protein Family
UniProt Gene Name
SULT1E1
UniProt Synonym Gene Names
STE; ST1E1
NCBI Protein Information
estrogen sulfotransferase

NCBI Summary for EST

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors. [provided by RefSeq, Jul 2008]
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UniProt Comments for EST

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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