• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Cardiac Myosin Heavy Chain (CMHC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS588374

Myosin is a highly conserved, ubiquitously expressed protein that hydrolyzes ATR and this reaction provides the energy required for muscle contraction that interacts with actin to generate the force for cellular movements. It is a hexameric protein composed… More details

$485 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cardiac Myosin Heavy Chain (CMHC), Monoclonal Antibody
Full Product Name
Cardiac Myosin Heavy Chain Mouse Monoclonal (3-48_G5C7)
Product Gene Name
anti-CMHC antibody
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
3-48
Host
Mouse
Species Reactivity
Human, Rat, Mouse
Predicted: Canine, Bovine and Rabbit
Form/Format
Lyophilized protein G purified in PBS pH7.4 (Full IgG)
Specificity
This antibody recognizes alpha and beta type heavy chains of light meromyosin fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains.
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
223,097 Da
3D Structure
ModBase 3D Structure for P12883
Preservative
None
Applications Tested/Suitable for anti-CMHC antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-CMHC antibody
If diluted in 100muL: WB: 1:500; IHC: 1:300.

Immunohistochemistry (IHC) of anti-CMHC antibody

Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-4B and irrelevant NeuAc monoClonal antibody at low microscopical magnification. A: cMHC 3-48-labeled cardiomyocytes. FFPE section of human heart ventricle was submitted to HIAR condition with citrate buffer prior to immunostaining. Immunostaining was revealed following incubation with biotinylated goat anti-mouse IgG, 20 minutes at 20C and horse radish peroxidase-diaminobemzidine enzymatic reaction causing brown precipitate to appear atthe sites of primary antibody binding. B: Control immunostaining performed following HIAR condition on the same microscopical slide with NeuAc monoClonal antibody showing no immunostaining but presence of light background, emphasizing specificity of immunostaining in (A). Magnification x30 C: Fragment of the same slide as in (A) with heart ventricle cardiomyocytes immunostained (brown). Magnification x580
Mouse Anti-CMHC Monoclonal Antibody – Cat# MBS588374

Testing Data of anti-CMHC antibody

Mouse Anti-CMHC Monoclonal Antibody, Testing Data – Cat# MBS588374

Other Notes

Small volumes of anti-CMHC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CMHC purchase

MBS588374 is an antibody targeting CMHC. Also known as Cardiac Myosin Heavy Chain Mouse Monoclonal (3-48_G5C7). Reactive in Human, Rat, Mouse
Predicted: Canine, Bovine and Rabbit. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CMHC. It may not necessarily be applicable to this product.

NCBI GI #
29727
NCBI GeneID
4625
NCBI Accession #
CAA37068.1
UniProt Primary Accession #
P12883
UniProt Secondary Accession #
Q14836; Q14837; Q14904; Q16579; Q2M1Y6; Q92679; Q9H1D5; Q9UDA2; Q9UMM8; A2TDB6; B6D424
UniProt Related Accession #
P12883
NCBI Official Full Name
cardiac beta myosin heavy chain
NCBI Official Synonym Full Names
myosin heavy chain 7
NCBI Official Symbol
MYH7
NCBI Official Synonym Symbols
CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
UniProt Protein Name
Myosin-7
UniProt Synonym Protein Names
Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta
UniProt Gene Name
MYH7
UniProt Synonym Gene Names
MYHCB; MyHC-slow; MyHC-beta
NCBI Protein Information
myosin-7

NCBI Summary for CMHC

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for CMHC

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.

Research Articles on CMHC

1. Of the 52 hypertrophic cardiomyopathy patients 12 (23.1%) had MYH7 variants.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CMHC antibody

 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram

Diseases associated with anti-CMHC antibody

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >297 publications with CMHC and Heart Diseases
 Cardiovascular Diseases Antibodies  >296 publications with CMHC and Cardiovascular Diseases
 Cardiomyopathies Antibodies  >253 publications with CMHC and Cardiomyopathies
 Cardiomyopathy, Hypertrophic Antibodies  >247 publications with CMHC and Cardiomyopathy, Hypertrophic
 Hypertrophy Antibodies  >125 publications with CMHC and Hypertrophy
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >103 publications with CMHC and Cardiomyopathy, Hypertrophic, Familial
 Cardiomegaly Antibodies  >74 publications with CMHC and Cardiomegaly
 Cardiomyopathy, Dilated Antibodies  >62 publications with CMHC and Cardiomyopathy, Dilated
 Heart Failure Antibodies  >61 publications with CMHC and Heart Failure
 Hypertrophy, Left Ventricular Antibodies  >54 publications with CMHC and Hypertrophy, Left Ventricular

Organs/Tissues associated with anti-CMHC antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >229 publications with CMHC and Muscle
 Heart Antibodies  >215 publications with CMHC and Heart
 Embryonic Tissue Antibodies  >17 publications with CMHC and Embryonic Tissue
 Brain Antibodies  >13 publications with CMHC and Brain
 Eye Antibodies  >6 publications with CMHC and Eye
 Lung Antibodies  >5 publications with CMHC and Lung
 Thyroid Antibodies  >5 publications with CMHC and Thyroid
 Nerve Antibodies  >3 publications with CMHC and Nerve
 Testis Antibodies  >2 publications with CMHC and Testis
 Liver Antibodies  >2 publications with CMHC and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000