Transforming Growth Factor Beta 1 (TGF-b1) ELISA Kit

Intended Uses: This human lactoferrin assay is intended for the quantitative determination of lactoferrin in human plasma, serum, milk, and cell culture sample. For research use only.

Principle of the Assay: Human lactoferrin will bind to the affinity puriffied capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human lactoferrin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human lactoferrin and is measured along with the test sample. Color development is proportional to the concentration of total lactoferrin in the samples!!Background/Introduction: Lactoferrin [ aka lactotransferrin or growth-inhibiting protein 12] is an 82.4kDa 691 amino acid glycoprotein which carries 2 ferric ions per molecule and is the main iron binding protein in milk [1]. Lactoferrin has multiple physiologcial functions including iron absorption regulation and antibacterial, antiviral, and antiparasitic activity. Degranulated neutrophils are the primaty source of plasma lactoferrin which is increased during inflammation and bacterial infection [2].

Small volumes of TGF-b1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS590024 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transforming growth factor beta 1 (TGF-b1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGF-b1. The ELISA analytical biochemical technique of the MBS590024 kit is based on TGF-b1 antibody-TGF-b1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGF-b1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGF-b1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).