Beta2-Microglobulin (B2M) ELISA Kit

Principle of the assay: This B2MG enzyme linked immunosorbent assay (ELISA) kit uses a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for B2MG. Standards or samples are added to the microtiter plate wells and B2MG, if present, will bind to the antibody pre-coated on the wells. A preparation of horseradish peroxidase (HRP)-conjugated B2MG antibody is added to each well. The conjugated antibody will bind to the B2MG immobilized on the plate after incubation. All unbound components will be removed by subsequent washing procedure. Next, 3, 3', 5, 5' tetramethyl-benzidine(TMB), a substrate for HRP is added to each well. The colorimetric reaction is proportional to the B2MG content in each well. The reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at the wavelength of 450nm +/- 2nm. In order to quantify the amount of B2MG present in the sample, this Human B2MG ELISA Kit includes a set of calibration standards (6 standards). The calibration standards should be assayed at the same time as the samples to allow the operator to produce a standard curve of Optical Density (O.D.) versus B2MG concentration (?g/mL). The concentration of B2MG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: Beta 2 microglobulin (B2MG) is initially discovered as a small serum protein of 12,000 Dalton. The small size allows B2MG to pass through the glomerular membrane, but it is normally completely reabsorbed in the proximal tubules. B2MG normally presents in minute amount, but increases its expressions, or adapts to the fibrillary configuration in certain pathological states. B2MG is found to be a subunit of class I major histocompatibility complex (MHC I) that is expressed on all nucleated cell excluding red blood cells. B2MG non-covalently links with the a3 domain of human leukocyte antigen (HLA) to form MHC I. Inside cytoplasm, MHC I binds to intracellular degraded foreign peptides, or self-peptides in some conditions, through the a1 and a2 variable domains of HLA. Subsequently, MHC I is anchored to the cell surface through the a3 domain of HLA. By way of this procedure, MHC I presents the engulfed foreign peptides to (CD8+) cytotoxic T-cell, thus, plays an important role in cell-mediated immunity. When binding with HFE protein, B2MG was found to increase iron uptake. B2MG is also associated with MHC I - like molecules such as CD1 and Qa. This immunoassay is a tool for investigation of the function and regulation of the protein.

Small volumes of B2M elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS590050 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Beta2-Microglobulin (B2M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing B2M. The ELISA analytical biochemical technique of the MBS590050 kit is based on B2M antibody-B2M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect B2M antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, B2M. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).