Mouse Anti-PD-L1 Monoclonal Antibody – Cat# MBS590085

PD-L1 Antibody – Mouse Monoclonal

Name: PD-L1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS590085
Price: 610 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS590085

PD-L1, a 40KD transmembrane protein, is a ligand of immune checkpoint protein Programmed Death-1 (PD-1). During T-cell receptor signalling, the binding of PD-L1 to PD-1 transmits an inhibitory signal to activated T-cells to decrease their proliferation. PD-L1… More details

$610 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PD-L1, Monoclonal Antibody

Full Product Name

mAb Anti Human PD-L1 clone 12B4, Tracer (Biotin) (Monoclonal)

Product Gene Name

anti-PD-L1 antibody

Product Synonym Names

mAb anti-Human PD-L1; Mouse monoclonal antibody (mAb) to human programmed death-ligand 1 (PD-L1); mouse mAb to B7H1; mouse mAb to human CD274, Tracer (Biotin Labeled); Biotin conjugated Mouse monoclonal antibody (mAb) to human programmed death-ligand 1 (PD-L1)

Clonality

Monoclonal

Isotype

IgG1,k

Clone Number

12B4

Host

Mouse

Species Reactivity

Human. Others not tested.

Form/Format

Lyophilized from a solution in 0.01M PBS, pH 7.2

Purity/Purification

Protein G Affinity Purified

Specificity

Reactive with human programmed death-ligand 1 (PD-L1).

Preparation and Storage

Store at -20 degree C. Avoid repeated freeze/thaw cycles after reconstitution.

Molecular Weight

20,246 Da

Immunogen

Human cell expressed recombinant PDL-1 (Phe19-Arg238) with poly-histidine tag at C-terminus.

Light Chain

Kappa

Fusion Myeloma

Sp2/0-Ag14

Target Protein

Human programmed death-ligand 1 (PD-L1), also known as B7H1 or CD274.

Reconstitution

Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Note

Tracer antibody in match antibody pair, biotin conjugated antibody
Detection antibody in matched antibody pair, biotin conjugated.

Product Categories/Family for anti-PD-L1 antibody

Immunosuppression; Cancer Immunotherapy

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for anti-PD-L1 antibody

ELISA (EIA)

Application Notes for anti-PD-L1 antibody

Sandwich ELISA: Biotin labelled anti-PD-L1 clone 12B4, when used in combination with clone 15G3 (Cat. NO.: MBS592438) coated plate, can detect human cell expressed PD-L1 in Sandwich ELISA application
The antibody reacts with recombinant PD-L1 in indirect ELISA.

Other Notes

Small volumes of anti-PD-L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PD-L1 purchase

MBS590085 is an antibody targeting PD-L1. Also known as mAb Anti Human PD-L1 clone 12B4, Tracer (Biotin) (Monoclonal). Reactive in Human. Others not tested.. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PD-L1. It may not necessarily be applicable to this product.

NCBI GI #

930425329

NCBI GeneID

29126

NCBI Accession #

NP_001300958.1

UniProt Secondary Accession #

Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27

UniProt Related Accession #

Q9NZQ7

GenBank Nucleotide #

NM_001314029.1

NCBI Official Full Name

programmed cell death 1 ligand 1 isoform c

NCBI Official Synonym Full Names

CD274 molecule

NCBI Official Symbol

CD274

NCBI Official Synonym Symbols

B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

UniProt Protein Name

Programmed cell death 1 ligand 1

UniProt Synonym Protein Names

B7 homolog 11 PublicationManual assertion based on opinion iniRef.1"B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion."Dong H., Zhu G., Tamada K., Chen L.Nat. Med. 5:1365-1369(1999) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.

UniProt Gene Name

CD274

UniProt Synonym Gene Names

PD-L12 PublicationsManual assertion based on opinion iniRef.10; PDCD1 ligand 1; Programmed death ligand 1; B7-H11 PublicationManual assertion based on opinion iniRef.1

NCBI Protein Information

programmed cell death 1 ligand 1

NCBI Summary for PD-L1

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

UniProt Comments for PD-L1

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

Research Articles on PD-L1

1. an HBV-pSTAT3-SALL4-miR-200c axis regulates PD-L1 causing T cell exhaustion

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PD-L1 antibody

Reference Product	PubMed Publications
PDCD1 antibody	>520 publications with PD-L1 and PDCD1
CD80 antibody	>414 publications with PD-L1 and CD80
PDCD1LG2 antibody	>204 publications with PD-L1 and PDCD1LG2
HLA-DRB1 antibody	>1 publications with PD-L1 and HLA-DRB1
LCK antibody	>1 publications with PD-L1 and LCK
PTPN11 antibody	>1 publications with PD-L1 and PTPN11

Pathways associated with anti-PD-L1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
PD-1 Signaling Pathway antibodies	PD-1 Signaling Pathway Diagram

Diseases associated with anti-PD-L1 antibody

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>185 publications with PD-L1 and Lung Neoplasms
Lung Diseases Antibodies	>171 publications with PD-L1 and Lung Diseases
Inflammation Antibodies	>169 publications with PD-L1 and Inflammation
Adenocarcinoma Antibodies	>140 publications with PD-L1 and Adenocarcinoma
Neoplasms, Experimental Antibodies	>117 publications with PD-L1 and Neoplasms, Experimental
Liver Diseases Antibodies	>112 publications with PD-L1 and Liver Diseases
Necrosis Antibodies	>101 publications with PD-L1 and Necrosis
Nervous System Diseases Antibodies	>99 publications with PD-L1 and Nervous System Diseases
Neoplasm Metastasis Antibodies	>88 publications with PD-L1 and Neoplasm Metastasis
Kidney Diseases Antibodies	>86 publications with PD-L1 and Kidney Diseases

Organs/Tissues associated with anti-PD-L1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1113 publications with PD-L1 and Blood
Lung Antibodies	>240 publications with PD-L1 and Lung
Lymph Node Antibodies	>110 publications with PD-L1 and Lymph Node
Skin Antibodies	>80 publications with PD-L1 and Skin
Brain Antibodies	>51 publications with PD-L1 and Brain
Pancreas Antibodies	>33 publications with PD-L1 and Pancreas
Stomach Antibodies	>28 publications with PD-L1 and Stomach
Placenta Antibodies	>23 publications with PD-L1 and Placenta
Prostate Antibodies	>18 publications with PD-L1 and Prostate
Mouth Antibodies	>15 publications with PD-L1 and Mouth