Ebola-Z Glycoprotein Recombinant Protein

Ebola virus causes a severe hemorrhagic fever with 50-90% mortality. The EBOV glycoprotein is the only protein over the viron surface, it forms trimeric complex on viron surface, this trimeric complexes are the receptors for fusion and entry to host cell. Mucin like domain within EBOV glycoprotein contains multiple glycosylated amino acids, mucin like domain usually is the target recognized by its specific antibody. GP-mucin deficient in post-translational modification is comparable to the eukaryotic cell - expressed GP ectodomains in assay performance. New published article in Clinical vaccine and immunology 2014 showed that recombinant mucin like domain is a useful antigen to test specific antibodies from ebola virus infected patients. A mucin like domain containing 180 amino acids was derived from Zaire ebolavirus (strain Kikwit-95) gp mucin sequence, the expressed protein forms dimer, minic its natural status of trimeric complex on viron surface with the molecular weight 38kDa. The protein was expressed and purified from E Coli, a 6 x His tag is attached to its C terminus. Some times, virus like particle can be found from over 30% E Coli expressed viral antigen, multiple antigens can aggregate into one large particle, even though with reducing condition, multiplized proteins, such as dimer, trimer is still present on SDS-PAGE. Aggregated virus like particle has strong ability in immunal reaction, they can be used for vaccine study or generate better result in immunoassay for identification.

Small volumes of Ebola-Z glycoprotein recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS596083 is a recombinant protein targeting Ebola-Z glycoprotein. Also known as Virus like particle formed by recombinant Ebola-Z glycoprotein. Host: E Coli.