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Nitrogen Permease Regulator 2-Like Protein (NPRL2) Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6000028

MBS6000028 is an antibody targeting NPRL2. Also known as Nitrogen Permease Regulator 2-like Protein (NPR2-like Protein, NPRL2, Gene 21 Protein, G21 Protein, Tumor Suppressor Candidate 4, TUSC4). Reactive in Human. Host: Mouse. More details

$880 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nitrogen Permease Regulator 2-like Protein (NPRL2), Polyclonal Antibody
Full Product Name
Nitrogen Permease Regulator 2-like Protein (NPR2-like Protein, NPRL2, Gene 21 Protein, G21 Protein, Tumor Suppressor Candidate 4, TUSC4)
Product Gene Name
Product Synonym Names
Anti -Nitrogen Permease Regulator 2-like Protein (NPR2-like Protein, NPRL2, Gene 21 Protein, G21 Protein, Tumor Suppressor Candidate 4, TUSC4)
Immunogen Sequence
MGSGCRIECI FFSEFHLTLG PKITYQVPED FISRELFDTV QVYIITKPEP QNKLITVTAM EKKLIGCPVC IEHKKYSRNA LLFNLGFVCD AQAKTCALEP IVKKLAGYLT TLELESSFVS MEESKQKLVP IMTILLEELN ASGRCTLPID ESNTIHLKVI EQRPDPPVAQ EYDVPVFTKD KEDFFNSQWD LTTQQILPYI DGFRHIQKIS AEADVELNLV RIAIQNLLYY GVVTLVSILQ YSNVYCPTPK VQDLVDDKSL QEACLSYVTK QGHKRASLRD VFQLYCSLSP GTTVRDLIGR HPQQLQHVDE RKLIQFGLMK NLIRRLQKYP VRVTREEQSH PARLYTGCHS YDEICCKTGM SYHELDERLE NDPNIIICWK
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human TUSC4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
43,658 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (50384918..50388486, complement). Location: 3p21.3
OMIM
607072
3D Structure
ModBase 3D Structure for Q8WTW4
Immunogen
Full-length human TUSC4, aa1-381 (AAH21984.1).
Applications Tested/Suitable for anti-NPRL2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-NPRL2 antibody
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml

Western Blot (WB) of anti-NPRL2 antibody

TUSC4 polyclonal antibody. Western Blot analysis of TUSC4 expression in human liver.
Mouse Anti-NPRL2 Polyclonal Antibody – Cat# MBS6000028

Western Blot (WB) of anti-NPRL2 antibody

Western Blot analysis of TUSC4 expression in transfected 293T cell line by TUSC4 polyclonal antibody. Lane 1: TUSC4 transfected lysate (41.91kD). Lane 2: Non-transfected lysate.
Mouse Anti-NPRL2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6000028

Immunofluorescence (IF) of anti-NPRL2 antibody

Immunofluorescence of purified antibody to TUSC4 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-NPRL2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6000028

Other Notes

Small volumes of anti-NPRL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NPRL2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nitrogen permease regulator 2-like protein
NCBI Official Synonym Full Names
nitrogen permease regulator-like 2 (S. cerevisiae)
NCBI Official Symbol
NPRL2
NCBI Official Synonym Symbols
NPR2; NPR2L; TUSC4
UniProt Protein Name
Nitrogen permease regulator 2-like protein
UniProt Synonym Protein Names
Gene 21 protein; G21 protein; Tumor suppressor candidate 4
UniProt Gene Name
NPRL2
UniProt Synonym Gene Names
TUSC4; NPR2-like protein; G21 protein
UniProt Entry Name
NPRL2_HUMAN
NCBI Protein Information
nitrogen permease regulator 2-like protein; G21 protein; 2810446G01Rik; gene 21 protein; NPR2-like protein; tumor suppressor candidate 4; homologous to yeast nitrogen permease (candidate tumor suppressor)

UniProt Comments for NPRL2

NPRL2: Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs. Belongs to the NPR2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Tumor suppressor

Chromosomal Location of Human Ortholog: 3p21.3

Molecular Function: protein binding; GTPase activator activity; protein kinase activity

Biological Process: negative regulation of kinase activity; protein amino acid phosphorylation; positive regulation of GTPase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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