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Mesothelin (MSLN) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600014

Mesothelin is a 40kD glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of… More details

$1135 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mesothelin (MSLN), Monoclonal Antibody
Full Product Name
Mesothelin
Product Gene Name
Product Synonym Names
Anti -Mesothelin
Clonality
Monoclonal
Isotype
IgG2A
Clone Number
8B35
Host
Rat
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Concentration
~0.5mg/ml (after reconstitution) (lot specific)
Specificity
Recognizes human mesothelin.
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
Chromosome: 16; NC_000016.9 (810765..818865). Location: 16p13.3
OMIM
601051
Immunogen
Recombinant protein corresponding to Glu296-Gly580 from human mesothelin, expressed in NS0 cells.
Applications Tested/Suitable for anti-MSLN antibody
ELISA (EIA), Flow Cytometry (FC)
Application Notes for anti-MSLN antibody
Suitable for use in Flow Cytometry and Direct ELISA.
Dilution: Flow Cytometry: 2.5ug/10e6 cells. Validated using HeLa human cervical carcinoma cells.
Optimal dilutions to be determined by the researcher.
Other applications have not been tested.

Flow Cytometry (FC) of anti-MSLN antibody

Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with M300-01C or isotype control antibody followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody.
Rat Anti-MSLN Monoclonal Antibody – Cat# MBS600014

Flow Cytometry (FC) of anti-MSLN antibody

Flow Cytometry analysis of HeLa human cell line|using M300-01C or isotype control antibody followed by Phycoerythrin-conjugated anti-rat IgG F(ab')2 secondary antibody.
Rat Anti-MSLN Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS600014

ELISA (EIA) of anti-MSLN antibody

Mesothelin knockout HeLa human cervical epithelial carcinoma cell line was stained with M3006-01C (filled histogram) or isotype control antibody (open histogram) followed by Anti-Rat IgG F(ab')2 Secondary Antibody (PE). No staining in the Mesothelin knockout HeLa cell line was observed.
Rat Anti-MSLN Monoclonal Antibody, ELISA (EIA) – Cat# MBS600014

Other Notes

Small volumes of anti-MSLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSLN purchase

MBS600014 is an antibody targeting MSLN. Also known as Mesothelin. Reactive in Human. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for MSLN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN
NCBI Official Synonym Symbols
MPF; SMRP
UniProt Protein Name
Mesothelin
UniProt Synonym Protein Names
CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor
Protein Family
UniProt Gene Name
MSLN
UniProt Synonym Gene Names
MPF; MPF
UniProt Entry Name
MSLN_HUMAN
NCBI Protein Information
mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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