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Microphthalmia (MITF) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600026

Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the… More details

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Product Overview

Online Datasheet in PDF
Product Name
Microphthalmia (Mitf), Monoclonal Antibody
Full Product Name
Microphthalmia (Transcription Factor, MiTF) (BSA & Azide Free)
Product Gene Name
anti-Mitf antibody
Product Synonym Names
Anti -Microphthalmia (Transcription Factor, MiTF) (BSA & Azide Free)
Immunogen Sequence
N-terminal
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F276
Host
Mouse
Species Reactivity
Canine, Human
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M3891-01
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
It does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.1 it reacts with both melanocytic and nonmelanocytic isoforms of Mi. Species Crossreactivity: human and canine. Does not react with mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
52-56kD doublet
Chromosome Location
Chromosome: 4; NC_005103.3 (194667979..194918737). Location: 4q34-q41
3D Structure
ModBase 3D Structure for O88368
Immunogen
N-terminal fragment of human Mi protein
Cellular Localization
Nuclear
Positive Control
501 Mel cells1. Melanoma

Product Categories/Family for anti-Mitf antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-Mitf antibody
Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
Application Notes for anti-Mitf antibody
Dilution: Gel Supershift (Order Ab at 1mg/ml)
Western Blotting (Not verified)
Immunohistochemistry (Formalin/paraffin: 4-8ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.

Immunohistochemistry (IHC) of anti-Mitf antibody

Formalin-fixed, paraffin-embedded human melanoma stained with MBS600026 using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.
Mouse Anti-MITF Monoclonal Antibody – Cat# MBS600026

Western Blot (WB) of anti-Mitf antibody

Western Blot
Mouse Anti-MITF Monoclonal Antibody, Western Blot (WB) – Cat# MBS600026

Other Notes

Small volumes of anti-Mitf antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mitf purchase

MBS600026 is an antibody targeting Mitf. Also known as Microphthalmia (Transcription Factor, MiTF) (BSA & Azide Free). Reactive in Canine, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Mitf. It may not necessarily be applicable to this product.

NCBI GI #
3319231
NCBI GeneID
25094
NCBI Accession #
AAC26170.1
UniProt Primary Accession #
O88368
UniProt Related Accession #
O88368
NCBI Official Full Name
microphthalmia associated transcription factor
NCBI Official Synonym Full Names
microphthalmia-associated transcription factor
NCBI Official Symbol
Mitf
UniProt Protein Name
Microphthalmia-associated transcription factor
Protein Family
Microphthalmia-associated transcription factor
UniProt Gene Name
Mitf
UniProt Entry Name
MITF_RAT
NCBI Protein Information
microphthalmia-associated transcription factor

NCBI Summary for Mitf

basic helix-loop-helix-leucine zipper transcription factor; may play a role in osteoclast function [RGD, Feb 2006]

UniProt Comments for Mitf

MITF: a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Cellular Component: protein complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; cell fate commitment; camera-type eye development; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; bone remodeling; regulation of cell proliferation; regulation of osteoclast differentiation; regulation of transcription, DNA-dependent; pigmentation; regulation of gene expression; melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; cell differentiation; negative regulation of apoptosis
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Research Articles on Mitf

1. We have characterized the biological role of a 23-aa domain derived from PIAS3 that induces apoptosis in these cells by inhibiting the transcriptional activity of both MITF and STAT3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Mitf antibody

 Reference Product  PubMed Publications
 Kit antibody  >116 publications with Mitf and Kit
 Sox10 antibody  >79 publications with Mitf and Sox10
 Mlana antibody  >49 publications with Mitf and Mlana
 Sox9 antibody  >13 publications with Mitf and Sox9
 Creb1 antibody  >12 publications with Mitf and Creb1
 Edn3 antibody  >12 publications with Mitf and Edn3
 Mapk1 antibody  >6 publications with Mitf and Mapk1
 Mapk3 antibody  >6 publications with Mitf and Mapk3
 Tp53 antibody  >5 publications with Mitf and Tp53

Pathways associated with anti-Mitf antibody

 Products by Pathway  Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram

Organs/Tissues associated with anti-Mitf antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >166 publications with Mitf and Eye
 Embryonic Tissue Antibodies  >58 publications with Mitf and Embryonic Tissue
 Heart Antibodies  >19 publications with Mitf and Heart
 Spleen Antibodies  >9 publications with Mitf and Spleen
 Placenta Antibodies  >4 publications with Mitf and Placenta
 Cervix Antibodies  >3 publications with Mitf and Cervix
 Small Intestine Antibodies  >2 publications with Mitf and Small Intestine
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