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UBA2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6000294

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SAE2 (UBA2), Polyclonal Antibody
Full Product Name
SAE2, CT (SUMO-activating Enzyme Subunit 2, Anthracycline-associated Resistance ARX, Ubiquitin-like 1-activating Enzyme E1B, UBA2, UBLE1B, HRIHFB2115)
Product Gene Name
Product Synonym Names
Anti -SAE2, CT (SUMO-activating Enzyme Subunit 2, Anthracycline-associated Resistance ARX, Ubiquitin-like 1-activating Enzyme E1B, UBA2, UBLE1B, HRIHFB2115)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human UBA2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
71,224 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (34919264..34960798). Location: 19q12
OMIM
613295
3D Structure
ModBase 3D Structure for Q9UBT2
Immunogen
Synthetic peptide corresponding to human UBA2 at C-terminal conjugated to KLH.

Product Categories/Family for anti-UBA2 antibody

Applications Tested/Suitable for anti-UBA2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-UBA2 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.25-0.5ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml

Immunohistochemistry (IHC) of anti-UBA2 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-UBA2 Polyclonal Antibody – Cat# MBS6000294

Western Blot (WB) of anti-UBA2 antibody

Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25ug/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-UBA2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6000294

Other Notes

Small volumes of anti-UBA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBA2 purchase

MBS6000294 is an antibody targeting UBA2. Also known as SAE2, CT (SUMO-activating Enzyme Subunit 2, Anthracycline-associated Resistance ARX, Ubiquitin-like 1-activating Enzyme E1B, UBA2, UBLE1B, HRIHFB2115). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBA2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SUMO-activating enzyme subunit 2
NCBI Official Synonym Full Names
ubiquitin-like modifier activating enzyme 2
NCBI Official Symbol
UBA2
NCBI Official Synonym Symbols
ARX; SAE2; HRIHFB2115
UniProt Protein Name
SUMO-activating enzyme subunit 2
UniProt Synonym Protein Names
Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B
UniProt Gene Name
UBA2
UniProt Synonym Gene Names
SAE2; UBLE1B
UniProt Entry Name
SAE2_HUMAN
NCBI Protein Information
SUMO-activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; ubiquitin-like 1-activating enzyme E1B; anthracycline-associated resistance ARX; UBA2, ubiquitin-activating enzyme E1 homolog

NCBI Summary for UBA2

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
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UniProt Comments for UBA2

SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; EC 6.3.2.19; Motility/polarity/chemotaxis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; cytosol; nucleus

Molecular Function: SUMO activating enzyme activity; protein binding; protein heterodimerization activity; metal ion binding; enzyme activator activity; transcription factor binding; ATP binding

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; SMT3-dependent protein catabolic process; post-translational protein modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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