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Neurotrophin Receptor, P75 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600056

MBS600056 is an antibody targeting Neurotrophin Receptor, p75. Reactive in Human, Monkey. Host: Mouse. More details

$1250 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Neurotrophin Receptor, p75, Monoclonal Antibody
Full Product Name
Neurotrophin Receptor, p75
Product Synonym Names
Anti -Neurotrophin Receptor, p75
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5H1
Host
Mouse
Species Reactivity
Human, Monkey
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes low affinity p75NTR. Species Crossreactivity: Human, monkey.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human melanoma cell line WM245
Applications Tested/Suitable for anti-Neurotrophin Receptor, p75 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Neutralization
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization.
Dilution: Immunohistochemistry: Reported to detect p75NTR in tissue section. Also used in immuno-electron microscopy.
Biological Blockade: Reported to block 125I-labeled NGF binding to its receptor.
Immunoblot Analysis: 0.1-1ug/ml of this lot detected p75NTR in RIPA lysates from HS294T cells.
Immunoprecipitation: 4ug of a previous lot immunoprecipitated p75NTR from 500ug of HS294T RIPA lysate.

Testing Data of anti-Neurotrophin Receptor, p75 antibody

Representative blot from a previous lot. HS294T cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p75NTR (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates p75 NTR (69kD).
Mouse Anti-Neurotrophin Receptor, P75 Monoclonal Antibody – Cat# MBS600056

Other Notes

Small volumes of anti-Neurotrophin Receptor, p75 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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