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Netrin G2a (NTNG2) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600088

Mouse Netrin G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin G2a is approximately 485aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a… More details

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Product Overview

Online Datasheet in PDF
Product Name
Netrin G2a (Ntng2), Monoclonal Antibody
Full Product Name
Netrin G2a
Product Gene Name
Product Synonym Names
Anti -Netrin G2a
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7H116
Host
Rat
Species Reactivity
Mouse
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse Netrin G2a in direct ELISAs and Western Blots. Does not crossreact with recombinant mouse Netrin G1a, Netrin 1 or Netrin 4.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Molecular Weight
66,166 Da
Chromosome Location
chromosome: 2; Location: 2 B; 2 18.0 cM
3D Structure
ModBase 3D Structure for Q8R4F1
Immunogen
Recombinant protein corresponding to aa18-504 from mouse Netrin G2a expressed in NSO cells (NP_598007).
Hybridoma
Mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Netrin G2a.

Product Categories/Family for anti-Ntng2 antibody

Applications Tested/Suitable for anti-Ntng2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Ntng2 antibody
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
Dilution: Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse Netrin G2a is ~3ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Netrin-G2a is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Immunohistochemistry: 25ug/ml detects Netrin G2a in frozen normal mouse brain tissue sections.

Other Notes

Small volumes of anti-Ntng2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ntng2 purchase

MBS600088 is an antibody targeting Ntng2. Also known as Netrin G2a. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Ntng2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Netrin-G2a
NCBI Official Synonym Full Names
netrin G2
NCBI Official Symbol
Ntng2
NCBI Official Synonym Symbols
Lmnt2; 2610016D08Rik; Ntng2
UniProt Protein Name
Netrin-G2
UniProt Synonym Protein Names
Laminet-2
Protein Family
UniProt Gene Name
Ntng2
UniProt Synonym Gene Names
Lmnt2
UniProt Entry Name
NTNG2_MOUSE
NCBI Protein Information
netrin-G2; laminet 2; laminet-2; OTTMUSP00000012657; OTTMUSP00000012658

NCBI Summary for Ntng2

The protein encoded by this gene belongs to a subclass of the netrin family called netrin-G proteins. Unlike classic netrins, which act as diffusible chemoattractants, netrin-Gs are glycosylphosphatidylinositol-anchored membrane proteins that interact with specific transmembrane proteins. In mouse, this gene is preferentially expressed in the cerebral cortex, habenular nucleus and superior colliculus. Knockout mutant mice display a lack of behavioral startle in response to acoustic stimuli. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
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UniProt Comments for Ntng2

NTNG2: Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites

Protein type: Cell development/differentiation; Membrane protein, GPI anchor

Cellular Component: anchored to plasma membrane; membrane; axon; plasma membrane

Molecular Function: protein binding

Biological Process: nervous system development; axonogenesis; multicellular organismal development; cell differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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