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med27 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6000924

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. CRSP8… More details

$970 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CRSP8 (med27), Polyclonal Antibody
Full Product Name
CRSP8 (P37 TRAP/SMCC/PC2 Subunit, Transcriptional Coactivator CRSP34, CRSP Complex Subunit 8, Cofactor Required For Sp1 Transcriptional Activation Subunit 8, Mediator Complex Subunit 27, Mediator Of RNA Polymerase II Transcription Subunit 27, MED27, CRSP3
Product Gene Name
anti-med27 antibody
Product Synonym Names
Anti -CRSP8 (P37 TRAP/SMCC/PC2 Subunit, Transcriptional Coactivator CRSP34, CRSP Complex Subunit 8, Cofactor Required For Sp1 Transcriptional Activation Subunit 8, Mediator Complex Subunit 27, Mediator Of RNA Polymerase II Transcription Subunit 27, MED27, CRSP3
Immunogen Sequence
MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK TPLYSQLLQA YKWSNKLQYH AGLASGLLNQ QSLKRSANQM GVSAKRRPKA QPTTLVLPPQ YVDDVISRID RMFPEMSIHL SRPNGTSAML LVTLGKVLKV IVVMRSLFID RTIVKGYNEN VYTEDGKLDI WSKSNYQVFQ KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR TLEAFHDTCR Q
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rabbit
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human MED27.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
35,672 Da
Chromosome Location
Chromosome: 8; NC_007119.5 (12402202..12505608, complement). Location: chromosome: 8
3D Structure
ModBase 3D Structure for Q6PFL0
Immunogen
Full length human MED27, aa1-311 (NP_004260.2).

Product Categories/Family for anti-med27 antibody

Antibodies; Abs to Enzymes; Polymerase
Applications Tested/Suitable for anti-med27 antibody
Western Blot (WB)
Application Notes for anti-med27 antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-med27 antibody

MED27 rabbit polyclonal antibody. Western Blot analysis of MED27 expression in human kidney.
Rabbit Anti-med27 Polyclonal Antibody – Cat# MBS6000924

Western Blot (WB) of anti-med27 antibody

Western Blot analysis of MED27 expression in transfected 293T cell line by MED27 polyclonal antibody. Lane 1: MED27 transfected lysate (35.4kD). Lane 2: Non-transfected lysate.
Rabbit Anti-med27 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6000924

Other Notes

Small volumes of anti-med27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for med27 purchase

MBS6000924 is an antibody targeting med27. Also known as CRSP8 (P37 TRAP/SMCC/PC2 Subunit, Transcriptional Coactivator CRSP34, CRSP Complex Subunit 8, Cofactor Required For Sp1 Transcriptional Activation Subunit 8, Mediator Complex Subunit 27, Mediator Of RNA Polymerase II Transcription Subunit 27, MED27, CRSP3. Reactive in Human, Rabbit. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for med27. It may not necessarily be applicable to this product.

NCBI GI #
182891210
NCBI GeneID
393633
NCBI Accession #
AAI64095.1
UniProt Primary Accession #
Q6PFL0
UniProt Related Accession #
Q6PFL0
NCBI Official Full Name
Crsp8 protein
NCBI Official Synonym Full Names
mediator complex subunit 27
NCBI Official Symbol
med27
NCBI Official Synonym Symbols
crsp8; zgc:66302
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 27
UniProt Synonym Protein Names
Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27
Protein Family
Mediator of RNA polymerase II transcription
UniProt Gene Name
med27
UniProt Synonym Gene Names
crsp34; crsp8; CRSP complex subunit 8
UniProt Entry Name
MED27_DANRE
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 27; crsp34; trap37; CRSP complex subunit 8; cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa

UniProt Comments for med27

Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors

By similarity. Required for the development of dopaminergic amacrine cells in the retina. May also negatively regulate the development of rod photoreceptor cells. Ref.2

Subunit structure: Component of the Mediator complex

By similarity.

Subcellular location: Nucleus

By similarity.

Developmental stage: Maternally expressed. Ubiquitously expressed throughout development. Ref.2

Sequence similarities: Belongs to the Mediator complex subunit 27 family.
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Research Articles on med27

1. Ubiquitous expression of crsp34 mRNA may suggest a universal requirement for Crsp34 in transcriptional initiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-med27 antibody

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Organs/Tissues associated with anti-med27 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1 publications with med27 and Eye
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