Rabbit Anti-MLF1IP Polyclonal Antibody – Cat# MBS6000941

MLF1IP Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MLF1IP antibody detected the MLF1IP protein (arrow).

MLF1IP Antibody – Rabbit Polyclonal

Name: MLF1IP Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6000941
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6000941

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of… More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MLF1IP, Polyclonal Antibody

Full Product Name

MLF1IP, CT (MLF1IP, CENPU, ICEN24, KLIP1, PBIP1, Centromere protein U, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, centromere protein of 50kD)

Product Gene Name

anti-MLF1IP antibody

Product Synonym Names

Anti -MLF1IP, CT (MLF1IP, CENPU, ICEN24, KLIP1, PBIP1, Centromere protein U, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, centromere protein of 50kD)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

47,522 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (185615219..185655286, complement). Location: 4q35.1

OMIM

611511

3D Structure

ModBase 3D Structure for Q71F23

Immunogen

MLF1IP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the C-terminal region of human MLF1IP.

Product Categories/Family for anti-MLF1IP antibody

Antibodies; Abs to Centromeres

Applications Tested/Suitable for anti-MLF1IP antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-MLF1IP antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-MLF1IP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MLF1IP purchase

MBS6000941 is an antibody targeting MLF1IP. Also known as MLF1IP, CT (MLF1IP, CENPU, ICEN24, KLIP1, PBIP1, Centromere protein U, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, centromere protein of 50kD). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MLF1IP. It may not necessarily be applicable to this product.

NCBI GI #

146327058

NCBI GeneID

79682

NCBI Accession #

AAI41855.1

UniProt Primary Accession #

Q71F23

UniProt Secondary Accession #

Q32Q71; Q9H5G1; A2RRD9

UniProt Related Accession #

Q71F23

NCBI Official Full Name

MLF1IP protein

NCBI Official Synonym Full Names

MLF1 interacting protein

NCBI Official Symbol

MLF1IP

NCBI Official Synonym Symbols

CENPU; KLIP1; PBIP1; CENP50; CENPU50

UniProt Protein Name

Centromere protein U

UniProt Synonym Protein Names

Centromere protein of 50 kDa; CENP-50; Interphase centromere complex protein 24; KSHV latent nuclear antigen-interacting protein 1; MLF1-interacting protein; Polo-box-interacting protein 1

UniProt Gene Name

MLF1IP

UniProt Synonym Gene Names

CENPU; ICEN24; KLIP1; PBIP1; CENP-U; CENP-50

UniProt Entry Name

CENPU_HUMAN

NCBI Protein Information

centromere protein U; MLF1-interacting protein; centromere protein of 50 kDa; polo-box-interacting protein 1; interphase centromere complex protein 24; KSHV latent nuclear antigen interacting protein 1; KSHV latent nuclear antigen-interacting protein 1

UniProt Comments for MLF1IP

CENPU: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: nucleoplasm; microtubule organizing center; cytosol; nucleus

Biological Process: nucleosome assembly; chordate embryonic development; DNA replication-independent nucleosome assembly at centromere; viral reproduction; transcription, DNA-dependent; regulation of transcription, DNA-dependent; mitotic cell cycle

Research Articles on MLF1IP

1. Data propose that CENP-P/O/R/Q/U self-assembles on kinetochores with varying stoichiometry and undergoes a pre-mitotic maturation step that could be important for kinetochores switching into the correct conformation for microtubule-attachment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MLF1IP antibody

Reference Product	PubMed Publications
PLK1 antibody	>3 publications with MLF1IP and PLK1
NDC80 antibody	>1 publications with MLF1IP and NDC80

Pathways associated with anti-MLF1IP antibody

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Chromosome Maintenance Pathway antibodies	Chromosome Maintenance Pathway Diagram
Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies	Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Anaphase Pathway antibodies	Mitotic Anaphase Pathway Diagram
Mitotic Metaphase And Anaphase Pathway antibodies	Mitotic Metaphase And Anaphase Pathway Diagram
Mitotic Prometaphase Pathway antibodies	Mitotic Prometaphase Pathway Diagram
Nucleosome Assembly Pathway antibodies	Nucleosome Assembly Pathway Diagram
PLK1 Signaling Events Pathway antibodies	PLK1 Signaling Events Pathway Diagram

Diseases associated with anti-MLF1IP antibody

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>1 publications with MLF1IP and Brain Diseases
Nervous System Diseases Antibodies	>1 publications with MLF1IP and Nervous System Diseases
Disease Models, Animal Antibodies	>1 publications with MLF1IP and Disease Models, Animal
Cell Transformation, Neoplastic Antibodies	>1 publications with MLF1IP and Cell Transformation, Neoplastic
Adenocarcinoma Antibodies	>1 publications with MLF1IP and Adenocarcinoma
Neoplasms Antibodies	>1 publications with MLF1IP and Neoplasms

Organs/Tissues associated with anti-MLF1IP antibody

Organ/Tissue Name	Pubmed Publications
Thymus Antibodies	>1 publications with MLF1IP and Thymus
Kidney Antibodies	>1 publications with MLF1IP and Kidney
Brain Antibodies	>1 publications with MLF1IP and Brain
Bone Antibodies	>1 publications with MLF1IP and Bone
Bone Marrow Antibodies	>1 publications with MLF1IP and Bone Marrow
Liver Antibodies	>1 publications with MLF1IP and Liver
Uncharacterized Tissue Antibodies	>1 publications with MLF1IP and Uncharacterized Tissue
Testis Antibodies	>1 publications with MLF1IP and Testis