Glucocorticoid Receptor DNA Binding Factor 1 (ARHGAP35) Antibody – Mouse Monoclonal

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq]

GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor; GAPs, Rac/Rho; Transcription factor; GAPs

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleus; cytosol; actin cytoskeleton

Molecular Function: DNA binding; GTP binding; transcription corepressor activity

Biological Process: regulation of cell shape; axon guidance; regulation of small GTPase mediated signal transduction; camera-type eye development; transcription, DNA-dependent; negative regulation of Rho protein signal transduction; small GTPase mediated signal transduction; neural tube closure; forebrain development; negative regulation of vascular permeability; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent