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RPA70, P70 (RPA1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600132

MBS600132 is an antibody targeting RPA1. Also known as RPA70, p70 (Replication Protein A, RPA1, RP-A, REPA1, HSSB, Replication Protein A1 (70kD), RP-A, HSSB, Human Single Stranded DNA-Binding Protein, RF-A, Replication Factor A). Reactive in Human. Host:… More details

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Product Overview

Online Datasheet in PDF
Product Name
RPA70, p70 (RPA1), Monoclonal Antibody
Full Product Name
RPA70, p70 (Replication Protein A, RPA1, RP-A, REPA1, HSSB, Replication Protein A1 (70kD), RP-A, HSSB, Human Single Stranded DNA-Binding Protein, RF-A, Replication Factor A)
Product Gene Name
anti-RPA1 antibody
Product Synonym Names
Anti -RPA70, p70 (Replication Protein A, RPA1, RP-A, REPA1, HSSB, Replication Protein A1 (70kD), RP-A, HSSB, Human Single Stranded DNA-Binding Protein, RF-A, Replication Factor A)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C293
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human RPA70, p70.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
68,138 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (1733273..1802848). Location: 17p13.3
OMIM
179835
3D Structure
ModBase 3D Structure for P27694
Immunogen
Partial sequence of recombinant full-length protein to human RPA70, p70

Product Categories/Family for anti-RPA1 antibody

Antibodies; Abs to DNA Replication; Repair
Applications Tested/Suitable for anti-RPA1 antibody
Western Blot (WB)
Application Notes for anti-RPA1 antibody
Suitable for use in Western Blotting.
Dilution: Western Blotting: 1:1000-1:2000

Testing Data of anti-RPA1 antibody

Mouse Anti-RPA1 Monoclonal Antibody – Cat# MBS600132

Other Notes

Small volumes of anti-RPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPA1. It may not necessarily be applicable to this product.

NCBI GI #
4506583
NCBI GeneID
6117
NCBI Accession #
NP_002936.1
UniProt Primary Accession #
P27694
UniProt Secondary Accession #
Q59ES9; A8K0Y9
UniProt Related Accession #
P27694; Q7Z4G2
GenBank Nucleotide #
NM_002945.3
NCBI Official Full Name
replication protein A 70 kDa DNA-binding subunit
NCBI Official Synonym Full Names
replication protein A1, 70kDa
NCBI Official Symbol
RPA1
NCBI Official Synonym Symbols
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
UniProt Protein Name
Replication protein A 70 kDa DNA-binding subunit
UniProt Synonym Protein Names
Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein
Protein Family
DNA-directed RNA polymerase
UniProt Gene Name
RPA1
UniProt Synonym Gene Names
REPA1; RPA70
UniProt Entry Name
RFA1_HUMAN
NCBI Protein Information
replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; OTTHUMP00000115691; replication factor A protein 1; single-stranded DNA-binding protein

UniProt Comments for RPA1

RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: male germ cell nucleus; nucleoplasm; lateral element; PML body; DNA replication factor A complex; nucleus

Molecular Function: protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding

Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; homeostasis of number of cells within a tissue; double-strand break repair; transcription-coupled nucleotide-excision repair; positive regulation of cell proliferation; telomere maintenance via recombination; hemopoiesis; nucleotide-excision repair, DNA damage removal; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA-dependent DNA replication; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
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Research Articles on RPA1

1. data suggest that hnRNPA1, TERRA and POT1 act in concert to displace RPA from telomeric ssDNA after DNA replication, and promote telomere capping to preserve genomic integrity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPA1 antibody

 Reference Product  PubMed Publications
 XPA antibody  >59 publications with RPA1 and XPA
 PCNA antibody  >58 publications with RPA1 and PCNA
 RPA2 antibody  >47 publications with RPA1 and RPA2
 RAD51 antibody  >33 publications with RPA1 and RAD51
 RPA3 antibody  >24 publications with RPA1 and RPA3
 PRKDC antibody  >22 publications with RPA1 and PRKDC
 WRN antibody  >18 publications with RPA1 and WRN
 BLM antibody  >13 publications with RPA1 and BLM
 RAD52 antibody  >13 publications with RPA1 and RAD52
 ATRIP antibody  >10 publications with RPA1 and ATRIP

Pathways associated with anti-RPA1 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway antibodies  Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram

Diseases associated with anti-RPA1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >59 publications with RPA1 and Neoplasms
 Metabolic Diseases Antibodies  >50 publications with RPA1 and Metabolic Diseases
 Congenital Abnormalities Antibodies  >30 publications with RPA1 and Congenital Abnormalities
 Photosensitivity Disorders Antibodies  >23 publications with RPA1 and Photosensitivity Disorders
 Precancerous Conditions Antibodies  >23 publications with RPA1 and Precancerous Conditions
 Carcinoma Antibodies  >16 publications with RPA1 and Carcinoma
 Immune System Diseases Antibodies  >16 publications with RPA1 and Immune System Diseases
 Leukemia Antibodies  >9 publications with RPA1 and Leukemia
 Cardiovascular Diseases Antibodies  >9 publications with RPA1 and Cardiovascular Diseases
 Drug Toxicity Antibodies  >8 publications with RPA1 and Drug Toxicity

Organs/Tissues associated with anti-RPA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >18 publications with RPA1 and Blood
 Skin Antibodies  >7 publications with RPA1 and Skin
 Lung Antibodies  >4 publications with RPA1 and Lung
 Ovary Antibodies  >4 publications with RPA1 and Ovary
 Liver Antibodies  >4 publications with RPA1 and Liver
 Brain Antibodies  >4 publications with RPA1 and Brain
 Testis Antibodies  >3 publications with RPA1 and Testis
 Placenta Antibodies  >3 publications with RPA1 and Placenta
 Thymus Antibodies  >3 publications with RPA1 and Thymus
 Bone Antibodies  >3 publications with RPA1 and Bone
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