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MBOAT4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6002172

MBOAT4 mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. More details

$895 / 0.2 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MBOAT4, Polyclonal Antibody
Full Product Name
MBOAT4, ID (MBOAT4, GOAT, OACT4, Ghrelin O-acyltransferase, Membrane-bound O-acyltransferase domain-containing protein 4)
Product Gene Name
Product Synonym Names
Anti -MBOAT4, ID (MBOAT4, GOAT, OACT4, Ghrelin O-acyltransferase, Membrane-bound O-acyltransferase domain-containing protein 4)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,879 Da
Chromosome Location
Chromosome: 8; NC_000074.6 (34115030..34125185). Location: 8 A4; 8
3D Structure
ModBase 3D Structure for P0C7A3
Immunogen
MBOAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-287 amino acids from the Central region of human MBOAT4.
Applications Tested/Suitable for anti-Mboat4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Mboat4 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-Mboat4 antibody

MBOAT4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MBOAT4 antibody detected the MBOAT4 protein (arrow).
Rabbit Anti-MBOAT4 Polyclonal Antibody – Cat# MBS6002172

Western Blot (WB) of anti-Mboat4 antibody

Western blot analysis of MBOAT4 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse stomach tissue lysate. MBOAT4 (arrow) was detected using the purified Pab.
Rabbit Anti-MBOAT4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6002172

Immunohistochemistry (IHC) of anti-Mboat4 antibody

MBOAT4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBOAT4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MBOAT4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6002172

Other Notes

Small volumes of anti-Mboat4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mboat4 purchase

MBS6002172 is an antibody targeting Mboat4. Also known as MBOAT4, ID (MBOAT4, GOAT, OACT4, Ghrelin O-acyltransferase, Membrane-bound O-acyltransferase domain-containing protein 4). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Mboat4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Mboat4 protein
NCBI Official Synonym Full Names
membrane bound O-acyltransferase domain containing 4
NCBI Official Symbol
Mboat4
NCBI Official Synonym Symbols
GOAT; Gm171
UniProt Protein Name
Ghrelin O-acyltransferase
UniProt Synonym Protein Names
Membrane-bound O-acyltransferase domain-containing protein 4
UniProt Gene Name
Mboat4
UniProt Synonym Gene Names
Gm171; Goat; O-acyltransferase domain-containing protein 4
UniProt Entry Name
MBOA4_MOUSE
NCBI Protein Information
ghrelin O-acyltransferase; O-acyltransferase domain-containing protein 4; membrane-bound O-acyltransferase domain-containing protein 4

UniProt Comments for Mboat4

MBOAT4: Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Belongs to the membrane-bound acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, multi-pass; EC 2.3.1.-; Membrane protein, integral; Endoplasmic reticulum

Cellular Component: membrane; endoplasmic reticulum; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: keto acid formate lyase activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; S-malonyltransferase activity; 3-hydroxybutyryl-CoA thiolase activity; S-acetyltransferase activity; O-octanoyltransferase activity; O-sinapoyltransferase activity; acyl-CoA N-acyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; succinyltransferase activity; Ras palmitoyltransferase activity; palmitoyltransferase activity; C-acyltransferase activity; C-palmitoyltransferase activity; dihydrolipoamide S-acyltransferase activity; N-acetyltransferase activity; protein-cysteine S-acyltransferase activity; serine O-acyltransferase activity; malonyltransferase activity; octanoyltransferase activity; O-succinyltransferase activity; O-palmitoyltransferase activity; 3-ketopimelyl-CoA thiolase activity; carnitine O-acyltransferase activity; sterol O-acyltransferase activity; S-succinyltransferase activity; N-acyltransferase activity; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity; N-palmitoyltransferase activity; O-acyltransferase activity; acylglycerol O-acyltransferase activity; O-acetyltransferase activity; transferase activity; benzoyl acetate-CoA thiolase activity; sinapoyltransferase activity; S-acyltransferase activity; protein-cysteine S-myristoyltransferase activity; myristoyltransferase activity; N-succinyltransferase activity; acetyltransferase activity; transferase activity, transferring acyl groups; L-2-aminoadipate N-acetyltransferase activity

Biological Process: peptidyl-serine octanoylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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