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Lysine, Acetylated Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6002257

MBS6002257 is an antibody targeting Lysine, Acetylated. Also known as Lysine, Acetylated (Acetyl-Lysine). Host: Rabbit. More details

$1195 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Lysine, Acetylated, Polyclonal Antibody
Full Product Name
Lysine, Acetylated (Acetyl-Lysine)
Product Synonym Names
Anti -Lysine, Acetylated (Acetyl-Lysine)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a liquid containing 0.05% sodium azide and 30% glycerol.
Purity/Purification
Serum
Serum
Specificity
Recognizes acetyl-lysine containing proteins including histones, from sodium butyrate or Trichostatin A treated cells, p53 acetylated in vitro by p300 or PCAF, and chemically acetylated BSA.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A mixture of chemically acetylated antigens

Product Categories/Family for anti-Lysine, Acetylated antibody

Antibodies; Abs to Amino Acids
Applications Tested/Suitable for anti-Lysine, Acetylated antibody
Western Blot (WB), Immunoprecipitation (IP), Gel Shift Assay (GS/EMSA)
Application Notes for anti-Lysine, Acetylated antibody
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Immunoblot Analysis: 1:1000-1:2000 dilution of this lot detected: acetylated histones in acid-extracted proteins from sodium butyrate (5mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A). Chemically acetylated BSA (10ng) in a dot blot assay. A 1:1000 dilution of the previous lot of the antibody detected: acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B). GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C)
Immunoprecipitation: 5ul of a previous lot immunoprecipitated in vitro acetylated recombinant p300.

Western Blot (WB) of anti-Lysine, Acetylated antibody

WB: 20ug of untreated (lane 1) or trichostatin A-treated (lane 2) Cos-1 cell lysate was probed with MBS6002257.
Rabbit Anti-Lysine, Acetylated Polyclonal Antibody – Cat# MBS6002257

Other Notes

Small volumes of anti-Lysine, Acetylated antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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