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P18ink4c Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600241

p18INK4c is a specific inhibitor of cdk4/cdk6 and is expressed during G1-to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase. More details

$1070 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p18INK4c, Monoclonal Antibody
Full Product Name
p18INK4c (BSA & Azide Free)
Product Synonym Names
Anti -p18INK4c (BSA & Azide Free)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F298
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See P1000-27.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human p18INK4c. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
18kD
Immunogen
Purified recombinant p18INK4c protein. Cellular Localization: Nuclear and cytoplasmic.
Positive Control
HeLa cells, Tonsil, skeletal muscle, heart and pancreas

Product Categories/Family for anti-p18INK4c antibody

Antibodies; Abs to Cancer Markers
Applications Tested/Suitable for anti-p18INK4c antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-p18INK4c antibody
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
Dilution: Immunoprecipitation: 1-2ug/mg. Co-precipitates cdk4. Use Protein G. For optimum results, immunoprecipitate with Western blot.
Western Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): 1:50 for 30 min at RT. Staining of formalin/paraffin tissues is required. Boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Western Blot (WB) of anti-p18INK4c antibody

Western Blot using MBS600241
Mouse Anti-P18ink4c Monoclonal Antibody – Cat# MBS600241

Western Blot (WB) of anti-p18INK4c antibody

Western Blot using MBS600241
Mouse Anti-P18ink4c Monoclonal Antibody, Western Blot (WB) – Cat# MBS600241

Immunohistochemistry (IHC) of anti-p18INK4c antibody

Formalin-fixed, paraffin-embedded human tonsil stained with MBS600241 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.
Mouse Anti-P18ink4c Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS600241

Other Notes

Small volumes of anti-p18INK4c antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p18INK4c purchase

MBS600241 is an antibody targeting p18INK4c. Also known as p18INK4c (BSA & Azide Free). Reactive in Human, Mouse. Host: Mouse.
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