• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PPME1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6004392

Protein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit (PPP2CA; MIM 176915) (Ogris et al., 1999 [PubMed 10318862]). More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPME1, Polyclonal Antibody
Full Product Name
PPME1, CT (PPME1, PME1, Protein phosphatase methylesterase 1)
Product Gene Name
Product Synonym Names
Anti -PPME1, CT (PPME1, PME1, Protein phosphatase methylesterase 1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
39,142 Da
Chromosome Location
Chromosome: 1; NC_003070.9 (26343369..26345058, complement). Location: chromosome: 1
3D Structure
ModBase 3D Structure for Q84WM7
Immunogen
PPME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-379 amino acids from the C-terminal region of human PPME1.
Applications Tested/Suitable for anti-PPME1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PPME1 antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-PPME1 antibody

PPME1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PPME1 antibody detected the PPME1 protein (arrow).
Rabbit Anti-PPME1 Polyclonal Antibody – Cat# MBS6004392

Western Blot (WB) of anti-PPME1 antibody

Western blot analysis of PPME1 (arrow) using rabbit polyclonal PPME1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPME1 gene.
Rabbit Anti-PPME1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6004392

Other Notes

Small volumes of anti-PPME1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPME1 purchase

MBS6004392 is an antibody targeting PPME1. Also known as PPME1, CT (PPME1, PME1, Protein phosphatase methylesterase 1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PPME1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pectinesterase PPME1
NCBI Official Symbol
PPME1
NCBI Official Synonym Symbols
ATPPME1; PPME1; T17F3.3; T17F3_3
UniProt Protein Name
Pectinesterase PPME1
UniProt Synonym Protein Names
Pectin methylesterase 9; AtPME9; Pectin methylesterase PPME1; Protein POLLEN SPECIFIC PME 1
Protein Family
UniProt Gene Name
PPME1
UniProt Synonym Gene Names
ARATH9; AtPPME1; PE PPME1; AtPME9
UniProt Entry Name
PPME1_ARATH
NCBI Protein Information
pectinesterase PPME1

NCBI Summary for PPME1

Encodes a protein with pectin methylesterase activity. The protein expression was shown to be highly restricted to the pollen grain (no detection in any other tissues or in the pollen grains' surrounding cells of the anthers.

UniProt Comments for PPME1

Function: Acts in the modification of cell walls via demethylesterification of cell wall pectin. Involved in the pollen tube growth and determination of pollen tube morphology. Ref.5

Catalytic activity: Pectin + n H2O = n methanol + pectate.

Pathway: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.

Subunit structure: Interacts with PMEI2, and in vitro with PMEI1. Ref.7

Subcellular location: Endoplasmic reticulum. Golgi apparatus. Secreted › cell wall Ref.5 Ref.7.

Tissue specificity: Expressed in mature pollen grains in the anthers and on the stigma. Found in pollen tubes within the style. Located at the tip and in the shank of the pollen tube. Ref.5 Ref.6 Ref.7

Disruption phenotype: After germination, the pollen tube is sunted, curved and has an irregular morphology. No effect on the morphology of ungerminated pollen grains, on the efficiency of pollen germination, fertilization or seed production. Ref.5

Miscellaneous: The pectin methylesterase activity is inhibited by PMEI2.

Sequence similarities: Belongs to the pectinesterase family.

Sequence caution: The sequence AAG52566.1 differs from that shown. Reason: Erroneous gene model prediction.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000