• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

3/5/2013 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6004478

MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]). More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
3/5/2013, Polyclonal Antibody
Full Product Name
MARCH5, NT (MARCH5, RNF153, E3 ubiquitin-protein ligase MARCH5, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, Mitochondrial ubiquitin ligase, RING finger protein 153)
Product Synonym Names
Anti -MARCH5, NT (MARCH5, RNF153, E3 ubiquitin-protein ligase MARCH5, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, Mitochondrial ubiquitin ligase, RING finger protein 153)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MARCH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-41 amino acids from the N-terminal region of human MARCH5.

Product Categories/Family for anti-3/5/2013 antibody

Antibodies; Abs to Ubiquitin Ligases
Applications Tested/Suitable for anti-3/5/2013 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-3/5/2013 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-3/5/2013 antibody

Western blot analysis of MARCH5 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane).MARCH5 (arrow) was detected using the purified Pab.
Rabbit Anti-3/5/2013 Polyclonal Antibody – Cat# MBS6004478

Immunohistochemistry (IHC) of anti-3/5/2013 antibody

MARCH5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MARCH5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-3/5/2013 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6004478

Flow Cytometry (FC/FACS) of anti-3/5/2013 antibody

MARCH5 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-3/5/2013 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6004478

Other Notes

Small volumes of anti-3/5/2013 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 3/5/2013 purchase

MBS6004478 is an antibody targeting 3/5/2013. Also known as MARCH5, NT (MARCH5, RNF153, E3 ubiquitin-protein ligase MARCH5, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, Mitochondrial ubiquitin ligase, RING finger protein 153). Reactive in Human, Mouse. Host: Rabbit.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000