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CDK5RAP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6005042

MBS6005042 is an antibody targeting CDK5RAP1. Also known as CDK5RAP1, CT (CDK5RAP1, C20orf34, CDK5 regulatory subunit-associated protein 1, CDK5 activator-binding protein C42). Reactive in Human. Host: Rabbit. Specifically inhibits CDK5 activation by CDK5R1. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDK5RAP1, Polyclonal Antibody
Full Product Name
CDK5RAP1, CT (CDK5RAP1, C20orf34, CDK5 regulatory subunit-associated protein 1, CDK5 activator-binding protein C42)
Product Gene Name
Product Synonym Names
Anti -CDK5RAP1, CT (CDK5RAP1, C20orf34, CDK5 regulatory subunit-associated protein 1, CDK5 activator-binding protein C42)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
67,689 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (31946645..31989375, complement). Location: 20q11.21
OMIM
608200
3D Structure
ModBase 3D Structure for Q96SZ6
Immunogen
CDK5RAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 550~580 amino acids from the C-terminal region of human CDK5RAP1.
Applications Tested/Suitable for anti-CDK5RAP1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CDK5RAP1 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-CDK5RAP1 antibody

Western blot analysis of CDK5RAP1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDK5RAP1 (arrow) was detected using the purified Pab.
Rabbit Anti-CDK5RAP1 Polyclonal Antibody – Cat# MBS6005042

Immunohistochemistry (IHC) of anti-CDK5RAP1 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5RAP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CDK5RAP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6005042

Flow Cytometry (FC/FACS) of anti-CDK5RAP1 antibody

CDK5RAP1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CDK5RAP1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6005042

Other Notes

Small volumes of anti-CDK5RAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDK5RAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CDK5 regulatory subunit-associated protein 1 isoform d
NCBI Official Synonym Full Names
CDK5 regulatory subunit associated protein 1
NCBI Official Symbol
CDK5RAP1
NCBI Official Synonym Symbols
C42; CGI-05; HSPC167; C20orf34
UniProt Protein Name
CDK5 regulatory subunit-associated protein 1
UniProt Synonym Protein Names
CDK5 activator-binding protein C42
UniProt Gene Name
CDK5RAP1
UniProt Synonym Gene Names
C20orf34
UniProt Entry Name
CK5P1_HUMAN
NCBI Protein Information
CDK5 regulatory subunit-associated protein 1; CDK5 activator-binding protein C42

NCBI Summary for CDK5RAP1

This gene encodes a regulator of cyclin-dependent kinase 5 activity. This protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013]
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UniProt Comments for CDK5RAP1

CDK5RAP1: Specifically inhibits CDK5 activation by CDK5R1. Belongs to the methylthiotransferase family. MiaB subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: cytoplasm

Molecular Function: transferase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; protein complex binding; protein kinase binding

Biological Process: regulation of neuron differentiation; negative regulation of cyclin-dependent protein kinase activity; brain development; tRNA modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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