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Methionine Aminopeptidase 1 (METAP1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600512

Methionine Aminopeptidase 1 (MetAP-1) encodes a cytoplasmic protease that removes the NH 2-terminal Met from nascent proteins. More details

$1030 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Methionine Aminopeptidase 1 (METAP1), Monoclonal Antibody
Full Product Name
Methionine Aminopeptidase 1 (MetAP-1)
Product Gene Name
Product Synonym Names
Mouse anti-Human Methionine Aminopeptidase 1 (MetAP-1)
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7H228
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity/Purification
Purified by Protein G affinity chromatography.
Concentration
~0.5 mg/ml (after reconstitution) (lot specific)
Specificity
Recognizes human Methionine Aminopeptidase 1 (MetAP-1)in direct ELISAs and Western Blot. Does not react with recombinant human METAP2.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
43,215 Da
Chromosome Location
Chromosome: 4; NC_000004.11 (99916788..99983960). Location: 4q23
OMIM
610151
3D Structure
ModBase 3D Structure for P53582
Immunogen
Recombinant protein corresponding to aa52-386 of human Methionine Aminopeptidase 1 derived from Sterile-filtered21.
Species Sequence Homology
chimpanzee (99%), rat and mouse (98%), chicken (95%), drosophila (64%)
Grade
Affinity Purified

Product Categories/Family for anti-METAP1 antibody

Applications Tested/Suitable for anti-METAP1 antibody
ELISA (EL/EIA), Western Blot (WB)
Other applications not tested
Application Notes for anti-METAP1 antibody
Western Blot: 1-2ug/ml Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml The detection limit is ~3ng/well.

Optimal dilutions to be determined by the researcher

Other Notes

Small volumes of anti-METAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for METAP1 purchase

MBS600512 is an antibody targeting METAP1. Also known as Methionine Aminopeptidase 1 (MetAP-1). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for METAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
methionine aminopeptidase 1
NCBI Official Synonym Full Names
methionyl aminopeptidase 1
NCBI Official Symbol
METAP1
NCBI Official Synonym Symbols
MAP1A; MetAP1A; KIAA0094; DKFZp781C0419
UniProt Protein Name
Methionine aminopeptidase 1
UniProt Synonym Protein Names
Peptidase M 1
UniProt Gene Name
METAP1
UniProt Synonym Gene Names
KIAA0094
UniProt Entry Name
AMPM1_HUMAN
NCBI Protein Information
methionine aminopeptidase 1; MAP 1; metAP 1; peptidase M 1; OTTHUMP00000219920

UniProt Comments for METAP1

METAP1: a peptidase that removes the amino-terminal methionine from nascent proteins.

Protein type: Protease; EC 3.4.11.18

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: rhodopsin mediated signaling; regulation of translation; phototransduction, visible light; regulation of rhodopsin mediated signaling; peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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