Mouse Anti-CDKN2B Monoclonal Antibody – Cat# MBS6005476

Western Blot analysis of of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line lysates using MBS6005476 (1ug/ml). PVDF Membrane was probed using MBS6005476 (1ug/ml) followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for p15INK4b at approximately 15kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

P15ink4b (CDKN2B) Antibody – Mouse Monoclonal

Name: CDKN2B Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6005476
Price: 1045 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6005476

p15INK4B is an ~15kD member of the p16INK4 family. Human p15INK4B is 138aa in length and contains four ankyrin repeats, a common structural sequence found in members of the INK4 family. The first 50aa of p15INK4B share 44% sequence identity with p16INK4.… More details

$1045 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

p15INK4b (Cdkn2b), Monoclonal Antibody

Full Product Name

p15INK4b (Cyclin-dependent Kinase 4 Inhibitor B, Multiple Tumor Suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2)

Product Gene Name

anti-Cdkn2b antibody

Product Synonym Names

Anti -p15INK4b (Cyclin-dependent Kinase 4 Inhibitor B, Multiple Tumor Suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2)

Clonality

Monoclonal

Isotype

IgG2B

Clone Number

12K152

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Purity/Purification

Purified by Protein A affinity chromatography from hybridoma culture supernatant

Concentration

~0.5mg/mL (After reconstitution) (lot specific)

Specificity

Recognizes human p15INK4b. Species Crossreactivity: recombinant human p16INK4a (50%). Does not react with recombinant human p18INK4c or recombinant human p19INK4d.

Preparation and Storage

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chromosome Location

Chromosome: 4; NC_000070.6 (89306289..89311032, complement). Location: 4 C3-C6; 4 42.15 cM

3D Structure

ModBase 3D Structure for P42772

Immunogen

Recombinant corresponding to Arg2-Asp138 from human p15INK4b expressed in E.coli.

Grade

Affinity Purified

Product Categories/Family for anti-Cdkn2b antibody

Antibodies; Abs to Protein Kinase Inhibitors

Applications Tested/Suitable for anti-Cdkn2b antibody

Direct ELISA,, Western Blot and Immunocytochemistry. Other applications not tested.

Application Notes for anti-Cdkn2b antibody

Western Blot: 1ug/ml using 293T human embryonic cell line and Hela human cervical epithelial carcinoma cell line
Immunocytochemistry: 3-25ug/ml using immersion fixed HeLa human cervical epithelial carcinoma cell line
Optimal dilutions to be determined by the researcher.

Other Notes

Small volumes of anti-Cdkn2b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cdkn2b purchase

MBS6005476 is an antibody targeting Cdkn2b. Also known as p15INK4b (Cyclin-dependent Kinase 4 Inhibitor B, Multiple Tumor Suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Cdkn2b. It may not necessarily be applicable to this product.

NCBI GI #

1762920

NCBI GeneID

12579

NCBI Accession #

AAB39833.1

UniProt Primary Accession #

P42772

UniProt Related Accession #

P55271

NCBI Official Full Name

p15INK4B

NCBI Official Synonym Full Names

cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)

NCBI Official Symbol

Cdkn2b

NCBI Official Synonym Symbols

p15; MTS2; INK4b; AV083695; p15INK4b; p15(INK4b)

UniProt Protein Name

Cyclin-dependent kinase 4 inhibitor B

UniProt Synonym Protein Names

p14-INK4b; p15-INK4b

Protein Family

Cyclin-dependent kinase 4 inhibitor

UniProt Gene Name

Cdkn2b

UniProt Entry Name

CDN2B_MOUSE

NCBI Protein Information

cyclin-dependent kinase 4 inhibitor B; p14-INK4b; p15-INK4b; cyclin-dependent kinase inhibitor p15; cyclin-dependent kinase inhibitor protein; cyclin-dependent kinase inhibitor p15INK4b

UniProt Comments for Cdkn2b

p15-INK4B: a potent inhibitor that nteracts strongly with CDK4 and CDK6. Potential effector of TGF-beta induced cell cycle arrest.

Protein type: Inhibitor; Tumor suppressor; Protein kinase, regulatory subunit; Cell cycle regulation

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein kinase binding

Biological Process: spleen development; negative regulation of cell proliferation; mitotic cell cycle checkpoint; positive regulation of transforming growth factor beta receptor signaling pathway; cellular response to extracellular stimulus; negative regulation of phosphorylation; cellular response to nutrient; regulation of cyclin-dependent protein kinase activity; cell cycle; G2/M transition of mitotic cell cycle; cell cycle arrest; negative regulation of epithelial cell proliferation

Research Articles on Cdkn2b

1. data demonstrate in vivo that Mule suppresses Ras-mediated tumorigenesis by preventing an accumulation of c-Myc/Miz1 complexes that mediates p21 and p15 down-regulation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Cdkn2b antibody

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Signaling Pathway antibodies	Cell Cycle Signaling Pathway Diagram
Cyclin D Associated Events In G1 Pathway antibodies	Cyclin D Associated Events In G1 Pathway Diagram
G1 Phase Pathway antibodies	G1 Phase Pathway Diagram
G1 To S Cell Cycle Control Pathway antibodies	G1 To S Cell Cycle Control Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram

Diseases associated with anti-Cdkn2b antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>633 publications with Cdkn2b and Neoplasms
Leukemia Antibodies	>207 publications with Cdkn2b and Leukemia
Lymphoma Antibodies	>153 publications with Cdkn2b and Lymphoma
Chromosome Aberrations Antibodies	>104 publications with Cdkn2b and Chromosome Aberrations
Nervous System Diseases Antibodies	>84 publications with Cdkn2b and Nervous System Diseases
Cardiovascular Diseases Antibodies	>66 publications with Cdkn2b and Cardiovascular Diseases
Adenocarcinoma Antibodies	>62 publications with Cdkn2b and Adenocarcinoma
Glioma Antibodies	>60 publications with Cdkn2b and Glioma
Carcinoma, Squamous Cell Antibodies	>49 publications with Cdkn2b and Carcinoma, Squamous Cell
Cell Transformation, Neoplastic Antibodies	>44 publications with Cdkn2b and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-Cdkn2b antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>63 publications with Cdkn2b and Brain
Bone Marrow Antibodies	>59 publications with Cdkn2b and Bone Marrow
Lung Antibodies	>51 publications with Cdkn2b and Lung
Skin Antibodies	>35 publications with Cdkn2b and Skin
Stomach Antibodies	>15 publications with Cdkn2b and Stomach
Kidney Antibodies	>9 publications with Cdkn2b and Kidney
Spleen Antibodies	>8 publications with Cdkn2b and Spleen
Connective Tissue Antibodies	>5 publications with Cdkn2b and Connective Tissue
Intestine Antibodies	>4 publications with Cdkn2b and Intestine
Tongue Antibodies	>1 publications with Cdkn2b and Tongue