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UQCRFS1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6006070

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The transit peptide of the Rieske protein seems to form part… More details

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Product Overview

Online Datasheet in PDF
Product Name
UQCRFS1, Polyclonal Antibody
Full Product Name
UQCRFS1, CT (UQCRFS1, Cytochrome b-c1 complex subunit Rieske, mitochondrial, Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Cytochrome b-c1 complex subunit 11, Co
Product Gene Name
Product Synonym Names
Anti -UQCRFS1, CT (UQCRFS1, Cytochrome b-c1 complex subunit Rieske, mitochondrial, Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Cytochrome b-c1 complex subunit 11, Co
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
29,668 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (29698167..29704136, complement). Location: 19q12
OMIM
191327
3D Structure
ModBase 3D Structure for P47985
Immunogen
UQCRFS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the C-terminal region of human UQCRFS1.
Applications Tested/Suitable for anti-UQCRFS1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-UQCRFS1 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-UQCRFS1 antibody

UQCRFS1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UQCRFS1 antibody detected the UQCRFS1 protein (arrow).
Rabbit Anti-UQCRFS1 Polyclonal Antibody – Cat# MBS6006070

Immunohistochemistry (IHC) of anti-UQCRFS1 antibody

UQCRFS1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UQCRFS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-UQCRFS1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6006070

Flow Cytometry (FC/FACS) of anti-UQCRFS1 antibody

UQCRFS1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-UQCRFS1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6006070

Immunofluorescence (IF) of anti-UQCRFS1 antibody

Confocal immunofluorescent analysis of UQCRFS1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-UQCRFS1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6006070

Other Notes

Small volumes of anti-UQCRFS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UQCRFS1 purchase

MBS6006070 is an antibody targeting UQCRFS1. Also known as UQCRFS1, CT (UQCRFS1, Cytochrome b-c1 complex subunit Rieske, mitochondrial, Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Cytochrome b-c1 complex subunit 11, Co. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UQCRFS1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
UCRI_HUMAN
NCBI Official Synonym Full Names
ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
NCBI Official Symbol
UQCRFS1
NCBI Official Synonym Symbols
RIP1; RIS1; RISP; UQCR5
UniProt Protein Name
Cytochrome b-c1 complex subunit Rieske, mitochondrial
UniProt Synonym Protein Names
Complex III subunit 5; Cytochrome b-c1 complex subunit 5; Rieske iron-sulfur protein; RISP; Ubiquinol-cytochrome c reductase iron-sulfur subunitCleaved into the following chain:Cytochrome b-c1 complex subunit 11; Alternative name(s):; Complex III subunit IX; Ubiquinol-cytochrome c reductase 8 kDa protein
UniProt Gene Name
UQCRFS1
UniProt Synonym Gene Names
RISP
UniProt Entry Name
UCRI_HUMAN
NCBI Protein Information
cytochrome b-c1 complex subunit Rieske, mitochondrial; complex III subunit 5; Rieske iron-sulfur protein; cytochrome b-c1 complex subunit 5; ubiquinol-cytochrome c reductase iron-sulfur subunit

UniProt Comments for UQCRFS1

UQCRFS1: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.

Protein type: Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.10.2.2

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex III

Molecular Function: 2 iron, 2 sulfur cluster binding; metal ion binding; ubiquinol-cytochrome-c reductase activity; protein complex binding

Biological Process: response to drug; response to antibiotic; cellular metabolic process; response to hormone stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UQCRFS1 antibody

 Reference Product  PubMed Publications
 SDHB antibody  >1 publications with UQCRFS1 and SDHB
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